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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Eun-Kyung (Division of Animal Life Science and Bio/Molecular Informatics Center, Konkuk University) Lee, Na-Kyoung (Division of Animal Life Science and Bio/Molecular Informatics Center, Konkuk University) Lee, Si-Kyung (Department of Applied Biology and Chemistry, Konkuk University) Chang, Hyo-Ihl (School of Life Science and Biotechnology, Korea University) Paik, Hyun-Dong (Division of Animal Life Science and Bio/Molecular Informatics Center, Konkuk University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제30권 제4호
발행연도
2010.1
수록면
634 - 640 (7page)

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The principal objective of this study was to screen and select acid-tolerant Lactobacillus strains from chicken feces, feeds, and other sources. Fourty six strains evidencing acid tolerance (pH 3.5) were isolated in this study. Among them, nine strains exhibited marked immunostimulatory effects. Therefore, nine candidate strains were characterized for probiotic use. In order to evaluate macrophage activation, NO production was measured using RAW 264.7 cells. In particular, three strains (FC812, FC222, and FC113) evidenced the highest levels of NO production measured at $38.39{\pm}20.01,\;35.06{\pm}27.73$, and $33.88{\pm}15.99{\mu}M$, respectively, at a concentration of $10^{8}CFU/mL$. The majority of strains, with the exception of strain FC322, evidenced marked resistance to artificial gastric juice (pH 2.5 with 1%(w/v) pepsin). Additionally, strains FC222, FC421, FC511, and FC721 were highly resistant to artificial bile acid (0.1%(w/v) oxgall), whereas strains FC113, FC322, FC422, FC621, and FC812 were the least resistant to bile. All nine strains exerted antimicrobial effects against chickenrelated pathogens. Additionally, all nine strains were found to be resistant to several antibiotics. The isolated strains, except for strain FC322, were tentatively identified as Lactobacillus salivarius, using an API 50 CHL kit. These results demonstrate that some probiotic organisms may potentially probiotic properties, and thus may serve as an effective alternative to antibiotics in animal applications.

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