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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Cytoskeletal changes were observed during cell division of the green alga Zygnema cruciatum using flourescein isothiocynate (FITC)-conjugated phallacidin for F-actin staining and FITC-anti-$\alpha$-tubulin for microtubule staining. Z. cruciatum was uninucleate with two star-shaped chloroplasts. Nuclear division and cell plate formation occurred prior to chloroplast division. Actin filaments appeared on the chromosome and nuclear surface during prophase, and the F-actin ring appeared as the cleavage furrow developed. FITC-phallacidin revealed that actin filaments were attached to the chromosomes during metaphase. The F-actin ring disappeared at late metaphase. At telophase, FITC-phallacidin staining of actin filaments disappeared. FITC-anti-$\alpha$-tubulin staining revealed that microtubules were arranged beneath the protoplasm during interphase and then localized on the nuclear region at prophase, and that the mitotic spindle was formed during metaphase. The microtubules appeared between dividing chloroplasts. The results indicate that a coordination of actin filaments and microtubules might be necessary for nuclear division and chromosome movement in Z. cruciatum.
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참고문헌 (24)
참고문헌 신청
Bischoff, H. W. / 1963 / Phycological studies IV. Some soil algae from enchanted rock and related algal species / The University of Texas Publications :95
Cleary, A. L. / 1996 / Rearrangements of F-actin during stomatogenesis visualised by confocal microscopy in fixed and permeabilised Tradescentia leaf epidermis / Bot. Acta 109:15~24
Czaban, B. B. / 1994 / Rhodamine-phalloidin and anti-tubulin antibody staining of spindle fibres that were irradiated with an ultraviolet microbeam / Protoplasma 178:18~27
Garbary, D. J. / 1996 / Actin rings in cytokinesis of apical cells in red algae / Can. J. Bot. 74:971~974
Garbary, D. J. / 1996 / Fluorescent labelling of the cytoskeleton in Ceramium strictum (Rhodophyta) / J. Phycol. 32:85~93
Cleary, A. L. / 1996 / Rearrangements of F-actin during stomatogenesis visualised by confocal microscopy in fixed and permeabilised Tradescentia leaf epidermis / Bot. Acta 109:15~24
Czaban, B. B. / 1994 / Rhodamine-phalloidin and anti-tubulin antibody staining of spindle fibres that were irradiated with an ultraviolet microbeam / Protoplasma 178:18~27
Garbary, D. J. / 1996 / Actin rings in cytokinesis of apical cells in red algae / Can. J. Bot. 74:971~974
Garbary, D. J. / 1996 / Fluorescent labelling of the cytoskeleton in Ceramium strictum (Rhodophyta) / J. Phycol. 32:85~93
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