Antiproliferative Evaluation and Apoptosis Induction in MCF-7 Cells by Ziziphus spina christi Leaf Extracts

Farmani, Fatemeh; Moein, Mahmoodreza; Amanzadeh, Amir; Kandelous, Hirsa Mostafapour; Ehsanpour, Zahra; Salimi, Mona

추천

검색

자료유형: 학술저널

저자정보: Farmani, Fatemeh (Medicinal Plants Processing Research Center, School of Pharmacy, Shiraz University of Medical Sciences) Moein, Mahmoodreza (Medicinal Plants Processing Research Center, School of Pharmacy, Shiraz University of Medical Sciences) Amanzadeh, Amir (National Cell Bank of Iran, Pasteur Institute of Iran) Kandelous, Hirsa Mostafapour (Department of Physiology and Pharmacology, Pasteur Institute of Iran) Ehsanpour, Zahra (Department of Physiology and Pharmacology, Pasteur Institute of Iran) Salimi, Mona (Department of Physiology and Pharmacology, Pasteur Institute of Iran)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제17권 제1호

발행연도: 2016.1

수록면: 315 - 321 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background: Herbal medicine has becoming a potential source of treatment for different types of cancer including breast cancer. It has been shown that plants from the family Rhamnaceae possess anticancer activity. Objective: In this study, we determined the antiproliferative influence of Ziziphus spina christi- a species from this family- on the MCF-7 (human breast adenocarcinoma) cell line. Materials and Methods: The cytotoxicity of the total extract, ethanol, ethanol-aqueous (1:1) as well as aqueous fractions of Ziziphus spina christi leaves was evaluated through MTT assay against MCF-7 cell line. Cell cycle inhibition and apoptosis induction were assessed by flowcytometry cycle RNase/PI analysis and Annexin V-FLUOS, respectively. Apoptosis was also analyzed by immunoblotting assay. Results: Our results indicated that the ethanolic fraction had the lowest $IC_{50}$ value (0.02 mg/ml), induced cell cycle arrest at the G1/S phase as well as apoptosis after a 48h of treatment. Conclusions: This is the first report on anticancer effect of Ziziphus spina christi ethanolic fraction on breast cancer cells, providing a scientific basis for its utility in traditional medicine. However, further in-depth studies are needed to confirm the precise mechanisms.

#Ziziphus spina christi #anticancer influence #MCF-7 #cell cycling #apoptosis

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

Farmani, Fatemeh

소속기관 Medicinal Plants Processing Research Center, School of Pharmacy, Shiraz University of Medical Scienc