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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 784 - 789 (6page)
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초록· 키워드
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A heteropolysaccharide possessing strong inhibitory activity against cholesterol esterase, namely PS-A, was isolated from water extracts of Cordyceps sinensis by activity-guided fractionation. The molecular weight of PS-A was estimated as $4.6{\times}10^5$ Da. Compositional analysis revealed the presence of D-glucose, D-galactose, and D-mannose at a molar ratio of 2:1:1. The $^1H$ and $^{13}C$ nuclear magnetic resonance spectra, correlation spectroscopy, nuclear Overhauser effect spectroscopy, and heteronuclear multiple bond correlations experiment established the structure of PS-A as follows: ${\rightarrow}3){\alpha}-D-glcp(1{\rightarrow}3){\beta}-D-glcp(1{\rightarrow}3){\beta}-D-glcp(1{\rightarrow}$ ${{{{\frown\atop2}\atop\uparrow}\atop1}\atop\smile}$ ${\alpha}$-D-manp Results of the in vitro enzyme assay showed strong inhibition of cholesterol esterase by PS-A, with an $IC_{50}$ value of $12.7\;{\mu}g/mL$, suggesting the possibility of its usefulness as a potential agent for control of hypercholesterolemia.
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