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학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

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논문 기본 정보

자료유형
학술저널
저자정보
Karaoz, Erdal (Department of Histology & Embryology, Faculty of Medicine, Istinye University) Tepekoy, Filiz (Department of Histology & Embryology, Faculty of Medicine, Istinye University) Yilmaz, Irem (Center for Regenerative Medicine and Stem Cell Research & Manufacturing [LivMedCell]) Subasi, Cansu (Center for Regenerative Medicine and Stem Cell Research & Manufacturing [LivMedCell]) Kabatas, Serdar (Neurosurgery Clinic, Gaziosmanpasa Taksim Training and Research Hospital)
저널정보
대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제62권 제2호
발행연도
2019.1
수록면
153 - 165 (13page)

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Objective : Spinal cord injury (SCI) is a very serious health problem, usually caused by a trauma and accompanied by elevated levels of inflammation indicators. Stem cell-based therapy is promising some valuable strategies for its functional recovery. Nestin-positive progenitor and/or stem cells (SC) isolated from pancreatic islets (PI) show mesenchymal stem cell (MSC) characteristics. For this reason, we aimed to analyze the effects of rat pancreatic islet derived stem cell (rPI-SC) delivery on functional recovery, as well as the levels of inflammation factors following SCI. Methods : rPI-SCs were isolated, cultured and their MSC characteristics were determined through flow cytometry and immunofluorescence analysis. The experimental rat population was divided into three groups : 1) laminectomy & trauma, 2) laminectomy & trauma & phosphate-buffered saline (PBS), and 3) laminectomy+trauma+SCs. Green fluorescent protein (GFP) labelled rPI-SCs were transplanted into the injured rat spinal cord. Their motilities were evaluated with Basso, Beattie and Bresnahan (BBB) Score. After 4-weeks, spinal cord sections were analyzed for GFP labeled SCs and stained for vimentin, $S100{\beta}$, brain derived neurotrophic factor (BDNF), 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNPase), vascular endothelial growth factor (VEGF) and proinflammatory (interleukin [IL]-6, transforming growth factor $[TGF]-{\beta}$, macrophage inflammatory protein [MIP]-2, myeloperoxidase [MPO]) and anti-inflammatory (IL-1 receptor antagonis) factors. Results : rPI-SCs were revealed to display MSC characteristics and express neural and glial cell markers including BDNF, glial fibrillary acidic protein (GFAP), fibronectin, microtubule associated protein-2a,b (MAP2a,b), ${\beta}3$-tubulin and nestin as well as anti-inflammatory prostaglandin E2 receptor, EP3. The BBB scores showed significant motor recovery in group 3. GFP-labelled cells were localized on the injury site. In addition, decreased proinflammatory factor levels and increased intensity of anti-inflammatory factors were determined. Conclusion : Transplantation of PI-SCs might be an effective strategy to improve functional recovery following spinal cord trauma.
#Spinal cord #Wounds and injuries #Islets of langerhans #Stem cells #Regeneration

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참고문헌 (63)

참고문헌 신청
Abdullahi D / 2017 / Experimental spinal cord trauma: a review of mechanically induced spinal cord injury in rat models / Rev Neurosci 28:15~20 google schola Aggarwal S / 2005 / Human mesenchymal stem cells modulate allogeneic immune cell responses / Blood 105:1815~1822 google schola Ahuja CS / 2017 / Traumatic spinal cord injury-repair and regeneration / Neurosurgery 80(3S):S9~S22 google schola Aras Y / 2016 / The effects of adipose tissue-derived mesenchymal stem cell transplantation during the acute and subacute phases following spinal cord injury / Turk Neurosurg 26:127~139 google schola Basso DM / 1995 / A sensitive and reliable locomotor rating scale for open field testing in rats / J Neurotrauma 12:1~21 google schola

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