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자료유형
학술저널
저자정보
Kim, Sun-A (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) Lee, Mi-Kyoung (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) Yoo, Sang-Jin (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) Kim, Jae-Hwan (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) Lee, Hyung-Jae (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) Park, Kyoung-Sik (Nutrition & Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) Jeong, Ja-Young (Nutrition & Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) Kim, Hae-Yeong (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제53권 제2호
발행연도
2010.1
수록면
237 - 242 (6page)

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Duplex PCR analysis was developed to detect a genetically modified (GM) papaya event 55-1 in both fresh and processed papaya fruit. GM papaya event 55-1 is a genetically modified organism (GMO) not currently approved for food in Korea. Using a primer set specific to papain, an endogenous papaya gene, we obtained a PCR product only from papaya genomic DNA, thus defining the primer's specificity. In another qualitative PCR, we used an event-specific primer pair to amplify and distinguish the target region in GM papaya event 55-1 among fourteen different GM events. Combining the two primer pairs, we developed a duplex PCR and used it to detect GM papaya event 55-1 in eleven fresh and five processed food samples. This new duplex PCR method meets the need to monitor GM papaya event 55-1 in both fresh papaya and processed foods, such as dried or canned papaya.

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