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학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

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논문 기본 정보

자료유형
학술저널
저자정보
Han, Youngmin (Department of Neurosurgery, Kyungpook National University Hospital) Kim, Kyoung-Tae (Department of Neurosurgery, Kyungpook National University Hospital)
저널정보
대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제59권 제5호
발행연도
2016.1
수록면
437 - 441 (5page)

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Objective : Recently, regenerative therapies have been used in clinical trials (heart, cartilage, skeletal). We don't make use of these treatments to spinal cord injury (SCI) patients yet, but regenerative therapies are rising interest in recent study about SCI. Neural precursor/stem cell (NPSC) proliferation is a significant event in functional recovery of the central nervous system (CNS). However, brain NPSCs and spinal cord NPSCs (SC-NPSCs) have many differences including gene expression and proliferation. The purpose of this study was to investigate the influence of neural growth factor (NGF) on the proliferation of SC-NPSCs. Methods : NPSCs ($2{\times}10^4$) were suspended in $100{\mu}L$ of neurobasal medium containing NGF-7S (Sigma-Aldrich) and cultured in a 96-well plate for 12 days. NPSC proliferation was analyzed five times for either concentration of NGF (0.02 and 2 ng/mL). Sixteen rats after SCI were randomly allocated into two groups. In group 1 (SCI-vehicle group, n=8), animals received 1.0 mL of the saline vehicle solution. In group 2 (SCI-NGF group, n=8), the animals received single doses of NGF (Sigma-Aldrich). A dose of 0.02 ng/mL of NGF or normal saline as a vehicle control was intra-thecally injected daily at 24 hour intervals for 7 days. For Immunohistochemistry analysis, rats were sacrificed after one week and the spinal cords were obtained. Results : The elevation of cell proliferation with 0.02 ng/mL NGF was significant (p<0.05) but was not significant for 2 ng/mL NGF. The optical density was increased in the NGF 0.02 ng/mL group compared to the control group and NGF 2 ng/mL groups. The density of nestin in the SCI-NGF group was significantly increased over the SCI-vehicle group (p<0.05). High power microscopy revealed that the density of nestin in the SCI-NGF group was significantly increased over the SCI-vehicle group. Conclusion : SC-NPSC proliferation is an important pathway in the functional recovery of SCI. NGF enhances SC-NPSC proliferation in vitro and in vivo. NGF may be a useful option for treatment of SCI patients pending further studies to verify the clinical applicability.
#Neural growth factor #Spinal cord neural precursor/stem cell #Spinal cord injury

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참고문헌 (40)

참고문헌 신청
Ahmed S / 1995 / BDNF enhances the differentiation but not the survival of CNS stem cell-derived neuronal precursors / J Neurosci 15:5765~5778 google schola Al-Daccak R / 2015 / Allogenic benefit in stem cell therapy : cardiac repair and regeneration / Tissue Antigens 86:155~162 google schola Anderson DK / 1993 / Pathophysiology of spinal cord trauma / Ann Emerg Med 22:987~992 google schola Beattie MS / 2004 / Inflammation and apoptosis : linked therapeutic targets in spinal cord injury / Trends Mol Med 10:580~583 google schola Chen SQ / 2014 / Combined use of NGF/BDNF/bFGF promotes proliferation and differentiation of neural stem cells in vitro / Int J Dev Neurosci 38:74~78 google schola

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