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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Kyung-Bok (Department of Biochemistry, Konyang University) Song, Kyung-Sik (School of Applied Biosciences, Kyungpook National University) Moon, Eun-Ho (Bangmok College of Basic Studies, Myongji University) Lee, Jung-Lim (Department of Microbiology, Konyang University) Yoo, Yung-Choon (Department of Microbiology, Konyang University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제52권 제3호
발행연도
2009.1
수록면
234 - 240 (7page)

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초록· 키워드

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The adjuvant effect of two types of water extracts of Cudrania tricuspidata (CT), cold water extract (CT-CW) and hot water extract (CT-HW), on induction of humoral and cellular immune responses against keyhole limpet hemocyanine (KLH) was examined. When mice were immunized subcutaneously (s.c.) twice at 2-week interval with KLH (20 ${\mu}g/mouse$) admixed with or without 50 mg/mouse of CT-CW or CT-HW, mice immunized with KLH+CT-CW and KLH+CT-HW showed significantly higher antibody titers against KLH than those immunized with KLH alone. Increase of the number of KLH-specific antibody producing B lymphocytes was observed in mice immunized admixed with CT-CW and CT-HW. The assay for determining isotypes of antibodies showed that both CT-CW and CT-HW augmented KLH-specific antibody titers of IgG1, IgG2a and IgG2b. In an in vitro analysis of T lymphocyte proliferation to KLH on week 4, the splenocytes of both mice treated with KLH+CT-CW and KLH+CT-HW showed a significantly higher proliferating activity than those treated with KLH alone. And the culture supernatants of the cell cultures from both mice immunized admixed with CT-CW and CT-HW showed a higher level of KLH-specific IL-2 production. Furthermore, mice immunized twice with KLH+CT-CW and KLH+CT-HW and followed by intrafootpad (i.f.) injection of KLH (20 ${\mu}g/site$) 7 weeks after the primary immunization induced a higher delayed-type hypersensitivity (DTH) reaction than mice treated with KLH alone. These results suggest that CT possesses a potent immunoadjuvant activity to enhance antigen-specific immune responses.

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