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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Jae Pil (Department of Pharmacy, Sunchon National University) Seo, Gu-Won (Department of Agricultural Chemistry, Sunchon National University) An, Shin-Deuk (Department of Agricultural Chemistry, Sunchon National University) Kim, Hoon (Department of Pharmacy, Sunchon National University)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제60권 제3호
발행연도
2017.1
수록면
257 - 263 (7page)

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A cellulolytic strain Y2 was isolated from soil obtained in the Canadian Alpine region. The isolate was identified as Paenibacillus sp. Y2 by 16S rRNA sequencing. When grown in LB medium supplemented with carboxymethyl-cellulose (CMC), CMCase production increased to 122.0% of that observed in LB without CMC. Culture supernatant was concentrated by ultrafiltration and 80% ammonium sulfate precipitates were separated by Hi-Trap Q and CHT-II chromatography. The purified enzyme (EG-PY2) showed a homogeneous single band and the molecular mass was estimated to be 38 kDa by SDS-PAGE. Optimum pH and temperature of the enzyme were 4.5 and $30^{\circ}C$, respectively. The half-life of enzyme activity at 50 was 140.7 min, but the enzyme was drastically inactivated within 5 min at $55^{\circ}C$. The enzyme was highly activated to 135.7 and 126.7% by 5.0 mM of $Cu^{2+}$ or $Mg^{2+}$ ions, respectively, and moderately activated by $Ba^{2+}$ and $Ca^{2+}$ ions, whereas it was inhibited to 76.8% by $Fe^{2+}$, and to ${\leq}50%$ by $Mn^{2+}$, $Co^{2+}$, $Zn^{2+}$, and EDTA. The enzyme was activated to 211.5% in the presence of 0.5 M of NaCl and greatly tolerant to 3.15M of NaCl. The enzyme showed 2.98 times higher ${\beta}$-glucanase activity than CMCase activity. Based on these results, it can be concluded that EG-PY2 is an acidophilic, cold-active, and halotolerant endoglucanase. The authors suggest it is considered to be useful for various industrial applications, such as, fruit juice clarification, acidic deinking processes, high-salt food processing, textile and pulp industries, and for biofuel production from seaweeds.
#Acidophilic endoglucanase #Alpine region #Cold-active #Halotolerant #Metal ion activation #Paenibacillus sp #Y2

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참고문헌 (40)

참고문헌 신청
Álvarez C / 2016 / Enzymatic hydrolysis of biomass from wood / Microb Biotechnol 9:149~156 google schola Amore A / 2013 / Industrial waste based compost as a source of novel cellulolytic strains and enzymes / FEMS Microbiol Lett 339:93~101 google schola Anand AA / 2010 / Isolation and characterization of bacteria from the gut of Bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion / J Insect Sci 10:107 google schola Baek SC / 2017 / Improvement of enzyme activity of β-1,3-1,4-glucanase from Paenibacillus sp. X4 by error-prone PCR and structural insights of mutated residues / Appl Microbiol Biotechnol 101:4073~4083 google schola Bai X / 2016 / Cloning, expression and characterization of a cold-adapted endo-1, 4-β-glucanase from Citrobacter farmeri A1, a symbiotic bacterium of Reticulitermes labralis / PeerJ 4:E2679 google schola

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