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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 544 - 550 (7page)
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Objective : Cerebral endothelial cells have unique biological features and are fascinating candidate cells for stroke therapy. Methods : In order to understand the molecular mechanisms of human cerebral endothelial cell (hCMEC/D3) transplantation in a rat stroke model, we performed proteomic analysis using 2-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Protein expression was confirmed by quantitative real-time PCR and Western blot. Results : Several protein spots were identified by gel electrophoresis in the sham, cerebral ischemia (CI), and CI with hCMEC/D3 treatment cerebral ischemia with cell transplantation (CT) groups, and we identified 14 differentially expressed proteins in the CT group. Proteins involved in mitochondrial dysfunction (paraplegin matrix AAA peptidase subunit, SPG7), neuroinflammation (peroxiredoxin 6, PRDX6), and neuronal death (zinc finger protein 90, ZFP90) were markedly reduced in the CT group compared with the CI group. The expression of chloride intracellular channel 4 proteins involved in post-ischemic vasculogenesis was significantly decreased in the CI group but comparable to sham in the CT group. Conclusion : These results contribute to our understanding of the early phase processes that follow cerebral endothelial cell treatment in CI. Moreover, some of the identified proteins may present promising new targets for stroke therapy.
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참고문헌 (36)
참고문헌 신청
Bernardi P / 2015 / Commentary : SPG7 is an essential and conserved component of the mitochondrial permeability transition pore / Front Physiol 6:320
Bohman S / 2005 / Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis / J Biol Chem 280:42397~42404
Brea D / 2015 / Study of protein expression in peri-infarct tissue after cerebral ischemia / Sci Rep 5:12030
Calderone A / 2003 / Ischemic insults derepress the gene silencer REST in neurons destined to die / J Neurosci 23:2112~2121
Chalothorn D / 2009 / Chloride intracellular channel-4 is a determinant of native collateral formation in skeletal muscle and brain / Circ Res 105:89~98
Bohman S / 2005 / Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis / J Biol Chem 280:42397~42404
Brea D / 2015 / Study of protein expression in peri-infarct tissue after cerebral ischemia / Sci Rep 5:12030
Calderone A / 2003 / Ischemic insults derepress the gene silencer REST in neurons destined to die / J Neurosci 23:2112~2121
Chalothorn D / 2009 / Chloride intracellular channel-4 is a determinant of native collateral formation in skeletal muscle and brain / Circ Res 105:89~98
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