지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 407 - 413 (7page)
이용수
초록· 키워드
오류제보하기
Paraquat dichloride (N,N-dimethyl-4-4'-bipiridinium, PQ) is an extremely toxic chemical that is widely used in herbicides. PQ generates reactive oxygen species (ROS) and causes multiple organ failure. In particular, PQ has been reported to be an immunotoxic agrochemical compound. PQ was shown to decrease the number of macrophages in rats and suppress monocyte phagocytic activity in mice. However, the effect of PQ on macrophage cell viability remains unclear. In this study, we evaluated the cytotoxic effect of PQ on the mouse macrophage cell line, RAW264.7 and its possible mechanism of action. RAW264.7 cells were treated with PQ (0, 75, and $150{\mu}M$), and cellular apoptosis, mitochondrial membrane potential (MMP), and intracellular ROS levels were determined. Morphological changes to the cell nucleus and cellular apoptosis were also evaluated by DAPI and Annexin V staining, respectively. In this study, PQ induced apoptotic cell death by dose-dependently decreasing MMP. Additionally, PQ increased the cleaved form of caspase-3, an apoptotic marker. In conclusion, PQ induces apoptosis in RAW264.7 cells through a ROS-mediated mitochondrial pathway. Thus, our study improves our knowledge of PQ-induced toxicity, and may give us a greater understanding of how PQ affects the immune system.
목차
등록된 정보가 없습니다.
참고문헌 (40)
참고문헌 신청
Ali, S. / 1996 / Paraquat induced DNA damage by reactive oxygen species / Biochem. Mol. Biol. Int 39:63~67
Boxer, L. A. / 1979 / Regulation of membrane peroxidation in health and disease / Pediatrics 64:713~718
Cappelletti, G. / 1998 / Apoptosis in human lung epithelial cells: triggering by paraquat and modulation by antioxidants / Cell Biol. Int 22:671~678
Caroleo, M. C. / 1996 / Chronic administration of paraquat produces immunosuppression of T-lymphocytes and astrocytes in rats / Toxic Subst. Mech 15:183~194
Castello, P. R. / 2007 / Mitochondria are a major source of paraquat-induced reactive oxygen species production in the brain / J. Biol. Chem 282:14186~14193
Boxer, L. A. / 1979 / Regulation of membrane peroxidation in health and disease / Pediatrics 64:713~718
Cappelletti, G. / 1998 / Apoptosis in human lung epithelial cells: triggering by paraquat and modulation by antioxidants / Cell Biol. Int 22:671~678
Caroleo, M. C. / 1996 / Chronic administration of paraquat produces immunosuppression of T-lymphocytes and astrocytes in rats / Toxic Subst. Mech 15:183~194
Castello, P. R. / 2007 / Mitochondria are a major source of paraquat-induced reactive oxygen species production in the brain / J. Biol. Chem 282:14186~14193
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Toxicokinetics of paraquat in Korean patients with acute poisoning
The Korean Journal of Physiology & Pharmacology
2016 .01
-
Anti-tumor activities of Panax quinquefolius saponins and potential biomarkers in prostate cancer
Journal of Ginseng Research
2021 .03
-
Quinetides: diverse posttranslational modified peptides of ribonuclease-like storage protein from Panax quinquefolius as markers for differentiating ginseng species
Journal of Ginseng Research
2020 .09
-
Common Pesticides Used in Suicide Attempts Following the 2012 Paraquat Ban in Korea
Journal of Korean Medical Science
2015 .01
-
원위 요골 골절의 수술적 치료 후 방형회내근의 초음파 평가
대한골절학회지
2017 .01
-
Anticancer Activity of Bispidinone Derivative by Induction of Apoptosis
대한의생명과학회지
2020 .12
-
MS-5, a Naphthalene Derivative, Induces the Apoptosis of an Ovarian Cancer Cell CAOV-3 by Interfering with the Reactive Oxygen Species Generation
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Minimal systems analysis of mitochondria-dependent apoptosis induced by cisplatin
The Korean Journal of Physiology & Pharmacology
2016 .07
-
Recombinant Rv0753c Protein of Mycobacterium tuberculosis Induces Apoptosis Through Reactive Oxygen Species-JNK Pathway in Macrophages
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2020 .12
-
Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells
Journal of Ginseng Research
2018 .04
-
Validation of the Korean Version of the Prodromal Questionnaire-Brief Version in Non-Help-Seeking Individuals
PSYCHIATRY INVESTIGATION
2019 .01
-
Downregulation of Reactive Oxygen Species in Apoptosis
대한암예방학회지
2016 .01
-
Molecular Mechanisms of Apoptosis and Roles in Cancer Development and Treatment
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
The Protective Effects of N-Acetyl-L-cysteine on Cadmium-induced Cell Apoptosis in Rat Testis
대한의생명과학회지
2019 .12
-
In vitro oxidative stress measuring methods to investigate the relationship between mitochondrial toxicity and kidney injury
한국실험동물학회 학술발표대회 논문집
2021 .07
-
Inhibitory Effects of Schisandra chinensis Seed Extracts on Breast Cancer
대한의생명과학회지
2025 .03
-
Quercetin Down-regulates IL-6/STAT-3 Signals to Induce Mitochondrial-mediated Apoptosis in a Non-small-cell Lung-cancer Cell Line, A549
Journal of Pharmacopuncture
2015 .01
-
Shikonin Exerts Cytotoxic Effects in Human Colon Cancers by Inducing Apoptotic Cell Death via the Endoplasmic Reticulum and Mitochondria-Mediated Pathways
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Gartanin Induces Apoptosis in Oral Squamous Cell Carcinoma Cell Lines through Mitochondrial Signaling Pathway
대한구강해부학회지
2015 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0