Validation protocol for whole-body dosimetry in an agricultural exposure study

Lee, Jiho; Kim, Eunhye; Shin, Yongho; Lee, Jonghwa; Lee, Junghak; Maasfeld, Wolfgang; Kim, Jeong-Han

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자료유형: 학술저널

저자정보: Lee, Jiho (Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University) Kim, Eunhye (Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University) Shin, Yongho (Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University) Lee, Jonghwa (Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University) Lee, Junghak (Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University) Maasfeld, Wolfgang (Bayer CropScience) Kim, Jeong-Han (Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University)

저널정보: 한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제61권 제1호

발행연도: 2018.1

수록면: 125 - 130 (6page)

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Agricultural workers exposed to pesticides can experience adverse health impacts depending on toxicity and exposure amount. Whole-body dosimetry (WBD) is the most reliable, practical, and realistic method for measuring exposure. Since validation of analytical and experimental methodologies is critical for quantitative determination of exposure, we conducted a validation procedure to design an essential protocol for WBD exposure studies. Using the fungicide kresoxim-methyl, matrixmatched standards were prepared with various matrices including outer cloth, inner cloth, washing solution for gloves and hands, gauze, and glass fiber filter (IOM sampler) to determine the instrumental limit of quantitation for high-performance liquid chromatography (HPLC) (2 ng) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) (10 pg). Method limits of quantitation (MLOQ) were also set for HPLC (0.1 mg/L) and LC-MS/MS (0.005 mg/L). We observed good analysis repeatability (coefficient of variation < 6%), and the linearity of the calibration curves was reasonable ($r^2$ > 0.998) in the range of 0.001-10 mg/L in various matrices. Recovery tests were carried out at three levels of concentration (MLOQ, 10 MLOQ, and 100 MLOQ) and resulted in good recoveries (72.7-105.6%). We did not observe breakthrough of the compound in tests of holding capacity for glass fiber pesticide filters. The procedures established in the present study are applicable as an essential, comprehensive protocol for exposure assessment studies using WBD.

#Exposure #IOM sampler #Kresoxim-methyl #Method validation #Pesticide #Whole-body dosimetry

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