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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 189 - 196 (8page)
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초록· 키워드
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The heavy metals are released into the environment due to the activities such as meeting the increasing demand for energy, industrial activities, and agricultural pesticides. In many studies, the heavy metals have been proven to have genotoxic effects. As a result of burning the lignite coal in thermal power plants, the heavy metals of Cr, Fe, Mn, Cu, Pb, Cd, Zn, and Ni are spread into the environment within the ashes. In the present study, the gene expression levels were examined on the corn and wheat plants added with 500-m interval around the thermic power plant. For this purpose, the genes from 14-3-3 protein family, expression level of which increases under abiotic stress conditions, were analyzed. For the expression levels of plants, the $2^{-{\Delta}{\Delta}C_t}$ values were calculated and then compared to $2^{-{\Delta}{\Delta}C_t}$ values of ${\beta}$-actin gene, that is, the housekeeping gene. The heavy metal content analyses of the samples were carried out using ICP-MS, and it was determined that there were many heavy metals at higher amounts within the structure of samples having low level of gene expression. It has been understood that heavy metal stress causes a difference in gene expression level. The change introduced by heavy metal stress into the gene expression occurs in concrete in the translation products. The level of stress-induced gene expression, which is caused by heavy metals in the environment surrounding the plant, has been successfully determined by RT-PCR.
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