Anti-Proliferative Activities of Vasicinone on Lung Carcinoma Cells Mediated via Activation of Both Mitochondria-Dependent and Independent Pathways

Dey, Tapan; Dutta, Prachurjya; Manna, Prasenjit; Kalita, Jatin; Boruah, Hari Prasanna Deka; Buragohain, Alak Kumar; Unni, Balagopalan

추천

검색

자료유형: 학술저널

저자정보: Dey, Tapan (Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology) Dutta, Prachurjya (Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology) Manna, Prasenjit (Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology) Kalita, Jatin (Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology) Boruah, Hari Prasanna Deka (Biological Sciences and Technology Division, CSIR-North East Institute of Science and Technology) Buragohain, Alak Kumar (Centre for Biotechnology and Bioinformatics, Dibrugarh University) Unni, Balagopalan (Biological Sciences, Assam Downtown University)

저널정보: 한국응용약물학회 Biomolecules & Therapeutics(구 응용약물학회지) Biomolecules & therapeutics 제26권 제4호

발행연도: 2018.1

수록면: 409 - 416 (8page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Vasicinone, a quinazoline alkaloid from Adhatoda vasica Nees. is well known for its bronchodilator activity. However its anti-proliferative activities is yet to be elucidated. Here-in we investigated the anti-proliferative effect of vasicinone and its underlying mechanism against A549 lung carcinoma cells. The A549 cells upon treatment with various doses of vasicinone (10, 30, 50, $70{\mu}M$) for 72 h showed significant decrease in cell viability. Vasicinone treatment also showed DNA fragmentation, LDH leakage, and disruption of mitochondrial potential, and lower wound healing ability in A549 cells. The Annexin V/PI staining showed disrupted plasma membrane integrity and permeability of PI in treated cells. Moreover vasicinone treatment also lead to down regulation of Bcl-2, Fas death receptor and up regulation of PARP, BAD and cytochrome c, suggesting the anti-proliferative nature of vasicinone which mediated apoptosis through both Fas death receptors as well as Bcl-2 regulated signaling. Furthermore, our preliminary studies with vasicinone treatment also showed to lower the ROS levels in A549 cells and have potential free radical scavenging (DPPH, Hydroxyl) activity and ferric reducing power in cell free systems. Thus combining all, vasicinone may be used to develop a new therapeutic agent against oxidative stress induced lung cancer.

#Vasicinone #Antioxidant #Anti-proliferative #A549 cells

참고문헌 (39)

참고문헌 신청

Amin, A. H. / 1959 / A bronchodilator alkaloid (vasicinone) from Adhatoda vasica Nees / Nature 184:1317

Claeson, U. P. / 2000 / Adhatoda vasica: a critical review of ethnopharmacological and toxicological data / J. Ethnopharmacol. 72:1~20

Deb, L. / 2015 / Past, present and perspectives of Manipur traditional medicine: a major health care system available for rural population in the North-East India / J. Ethnopharmacol. 169:387~400

Dutta, P. / 2016 / Antioxidant potential of Vespa affinis L., a traditional edible insect species of North East India / PLoS ONE 11:e0156107

Fawole, O. A. / 2010 / Anti-inflammatory, anticholinesterase, antioxidant and phytochemical properties of medicinal plants used for pain-related ailments in South Africa / J. Ethnopharmacol. 127:235~241

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!