지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The amplification mechanism of short interfering RNAs (siRNAs) along the transgene sequence exists in RNA interference (RNAi). The RNA-dependent RNA polymerase synthesizes complementary RNAs by using the transgene mRNA as a template, and the secondary siRNAs are generated from the outside of primary RNAi target. Four independent RNAi vectors which produced primary siRNAs against distinct regions of the tobacco endoplasmic reticulum ${\omega}-3$ fatty acid desaturase gene (NtFAD3) were transiently expressed in leaves of the NtFAD3-overexpressed transgenic plants. Regardless of the RNAi vector used, the secondary NtFAD3 siRNAs were generated preferentially from the 3' downstream region of the transgene. Secondary siRNAs from the 5' upstream region adjacent to the annealing site of primary siRNAs accumulated under the detection level. Our results suggest that different regulatory mechanisms are involved in the spreading of RNA silencing into 5' upstream and 3' downstream regions of the target sequence, respectively.
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