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논문 기본 정보

자료유형
학술저널
저자정보
도호정 (자생한방병원 한방재활의학과) 이진호 (자생의료재단 척추관절연구소) 하인혁 (자생의료재단 척추관절연구소) 정화진 (자생의료재단 척추관절연구소) 이재웅 (자생의료재단 척추관절연구소) 김민정 (자생의료재단 척추관절연구소) 김은지 (자생의료재단 척추관절연구소) 이인희 (자생의료재단 척추관절연구소)
저널정보
한방재활의학과학회 한방재활의학과학회지 한방재활의학과학회지 제27권 제1호
발행연도
2017.1
수록면
19 - 25 (7page)

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Objectives Eucommiae cortex is one of the frequently used herbs for musculoskeletal disorders, with well-known anti-inflammatory effect. Meanwhile, powdered form of herbal drugs is more advantageous than decoction form, in that storing and taking is more convenient. Thus, the authors aimed to investigate whether the drying method affects the index compound level and anti-inflammatory effect of eucommiae cortex water extract. Methods Eucommiae cortex was extracted in distilled water at $100^{\circ}C$ for 3 hours, filtered, and then evaporated under vacuum. One half of the sample was freeze-dried at $-80^{\circ}C$. Another half of the sample was added with dextrin and then spray-dried at $180^{\circ}C$. To assess the possible change in index compound content, pinoresinol diglucoside was selected as the index marker. The content level of the index compound in various extract sample was quantified through high performance liquid chromatography. In addition, iNOS assay in LPS-induced RAW 264.7 cells was adopted to determine the anti-inflammatory effect of various extract samples. Furthermore, MTT assay was performed to confirm that the result of iNOS assay was not due to cytotoxicity. Results There was no significance difference in index compound content between extract samples obtained through two different drying methods. Anti-inflammatory activity of extract samples were similar at the matching concentration, regardless of the drying methods. Extract samples did not show any significant cytotoxicity. Conclusions Extract samples of eucommiae cortex were obtained through freeze-drying and spray-drying. Neither change in index compound content nor difference in anti-inflammatory activity was observed between drying methods.

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