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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 117 - 123 (7page)
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Objectives : Sinhyowoldo-san (SHWDS) is said to be a traditional medicine used for shigellosis, abdominal pain, diarrhea. But mechanism of SHWDS mediated-modulation of immune function is not sufficiently understood. To ascertain the molecular mechanisms of SHWDS 70% EtOH extract on pharmacological and biochemical actions in inflammation, we researched the effect of pro-inflammatory mediators in phorbol-12-myristate-13-acetate (PMA)+ A23187-activated human mast cell line (HMC-1). Methods : In the present research, cell viability was measured by MTS assay. pro-inflammatory cytokine production was measured by performing enzyme-linked immunosorbent assay (ELISA), reverse transcription polymerase chain reaction (RT-PCR), and western blot analysis to analyze the activation of mitogen-activated protein kinases (MAPKs), nuclear factor kappa-light-chain-enhancer of activated B cells ($NF-{\kappa}B$). The investigation focused on whether SHWDS inhibited the expressions of interleukin-6 (IL-6), interleukin-8 (IL-8), MAPKs and $NF-{\kappa}B$ in PMA+A23187-activated HMC-1 cells. Results : SHWDS has no cytotoxicity at measured concentration (50, 100, and $250{\mu}g/ml$). SHWDS ($250{\mu}g/ml$) inhibits pro-inflammatory cytokine expression in PMA+ A23187-activated HMC-1 cells. Moreover, SHWDS inhibited cyclooxygenase (COX)-2 expression. In activated HMC-1 cells, SHWDS suppressed phosphorylation of extracellular signal-regulated kinase (ERK 1/2) and c-jun N-terminal Kinase (JNK 1/2). Then, SHWDS suppressed activation of nuclear factor $NF-{\kappa}B$ in nuclear, degradation of IkB ${\alpha}$ in cytoplasm. Conclusions : We propose that SHWDS has an anti-inflammatory therapeutic potential, which may result from inhibition of ERK 1/2, JNK 1/2 phosphorylation and $NF-{\kappa}B$ activation, thereby decreasing the expression of pro-inflammatory genes.
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참고문헌 (28)
참고문헌 신청
Her J / 2005 / Naekyung-Pyun of Donguibogam / Yeogang :564
Her J. / 2005 / Naekyung-Pyun of Donguibogam / Yeogang :3068
김승택 / 2013 / 梔子의 부위별 Geniposide정량 및 항산화효능비교 / 대한본초학회지 28(4):17~23
손동화 / 2012 / β-Glucosidase 처리에 의한 치자추출물의 항염증 활성 증진 / 한국식품과학회지 44(3):331~336
황금희 / 2007 / 치자추출물의 Monoamine Oxidase 저해활성 / 생약학회지 38(2):108~112
Her J. / 2005 / Naekyung-Pyun of Donguibogam / Yeogang :3068
김승택 / 2013 / 梔子의 부위별 Geniposide정량 및 항산화효능비교 / 대한본초학회지 28(4):17~23
손동화 / 2012 / β-Glucosidase 처리에 의한 치자추출물의 항염증 활성 증진 / 한국식품과학회지 44(3):331~336
황금희 / 2007 / 치자추출물의 Monoamine Oxidase 저해활성 / 생약학회지 38(2):108~112
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