Reduce the Energy Loss in Ruminant; Using Raphanus Sativus Extracts to Mitigate Methane Emission

이신자; 최유영; 이수경; 이일동; 엄준식; 김현상; 김도형; 이성실

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자료유형: 학술저널

저자정보: 이신자 (Institute of Agriculture and Life Science & University-Centered Labs, Gyeongsang National University) 최유영 (Division of Applied Life Science [BK21 program] and Institute of Agriculture & Life Science [IALS], Gyeongsang National University) 이수경 (Institute of Agriculture and Life Science, Gyeongsang National University) 이일동 (Division of Applied Life Science [BK21 program] and Institute of Agriculture & Life Science [IALS], Gyeongsang National University) 엄준식 (Division of Applied Life Science [BK21 program] and Institute of Agriculture & Life Science [IALS], Gyeongsang National University) 김현상 (Division of Applied Life Science [BK21 program] and Institute of Agriculture & Life Science [IALS], Gyeongsang National University) 김도형 (Department of Animal Science, GyeongBuk Provincial College) 이성실 (Division of Applied Life Science [BK21], IALS, Gyeong- sang National University)

저널정보: 한국유기농업학회 한국유기농업학회지 한국유기농업학회지 제25권 제4호

발행연도: 2017.1

수록면: 917 - 930 (14page)

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This study was conducted to evaluate Raphanus sativus extracts to methane reduction in rumen. Five different levels of R. sativus extracts were used to investigate the most effective dosing level for the decrease of methane production in the rumen. The rumen fluid was collected from a cannulated one Hanwoo cow ($BW=450{\pm}30kg$) consuming 600 g/kg timothy and 400 g/kg concentrate. On fermentation day, rumen fluid was collected at 2 hr postfeeding R. sativus extracts was dosed to achieve final concentration of 0, 1, 3, 5, 7, and 9% respectively, to fermentation bottles containing the mixture of rumen fluid and McDougall's buffer and 300 mg of timothy was added as a substrate. The fermentation was conducted for 3, 6, 9, 12, 24, 48 and 72 hr incubation time at $39^{\circ}C$ with shaking. In vitro ruminal pH values were measured normal range for ruminal fermentation. Dry matter disappearance was significantly higher (p<0.05) at 3 hr incubation time 1, 3 and 5% doses than that of control. The highest methane reduction was observed in 12 hr incubation time 5, 7 and 9%. The carbon dioxide emission was also significantly (p<0.05) lower than that of control at 12 hr incubation time 5, 7 and 9%. The total volatile fatty acid was no significant difference between control and all doses level at 12 and 24 hr incubation time. At 24 hr incubation time, the result of real-time PCR were indicated that M. archea was significantly lower (p<0.05) at all doses level comparing to that of control. In conclusion, R. sativus extracts were significantly decreased methane emission. R. sativus extracts were significantly lower (p<0.05) than that of control at 12 hr incubation time 5, 7 and 9% and no adversely effect in rumen pH, dry matter disappearance and total VFA.

#raphanus sativus #rumen fermentation #rumen methane emission

참고문헌 (11)

참고문헌 신청

Aderbal, M. D. A. / 2015 / Potential in vitro degradability and gas production of the byproducts of the biodiesel chain / Ciencia e Investigación Agraria 42(2):285~293

조은혜 / 2009 / 무의 α-Amylase 활성 및 가공 안정성 / 한국식품영양과학회지 38(6):812~815

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Koike, s / 2001 / Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens / FEMS microbiology letters 204(2):361~366

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최유영

소속기관 Division of Applied Life Science [BK21 program] and Institute of Agriculture & Life Science [IAL