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논문 기본 정보

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학술저널
저자정보
Kim, Bo-Hyun (Department of Biological Science, College of Natural Sciences, Wonkwang University) Jung, Ji-Ung (Department of Biological Science, College of Natural Sciences, Wonkwang University) Ko, Ki-Sung (Department of Biological Science, College of Natural Sciences, Wonkwang University) Kim, Won-Sin (Department of Biological Science, College of Natural Sciences, Wonkwang University) Kim, Sun-Mi (Department of Biological Science, College of Natural Sciences, Wonkwang University) Ryu, Jae-Sung (Department of Biological Science, College of Natural Sciences, Wonkwang University) Jin, Jung-Woo (Department of Biological Science, College of Natural Sciences, Wonkwang University) Yang, Hyo-Jung (Department of Biological Science, College of Natural Sciences, Wonkwang University) Kim, Ji-Su (Department of Biological Science, College of Natural Sciences, Wonkwang University) Kwon, Hyuck-Chan (Obstetric and gynecology clinic, Mirae-Heemang Hospital) Nam, Sang-Yoon (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) Kwak, Dong-Hoon (College of Veterinary Medicine and Research Institute of Veterinary) Park, Yong-Il Koo, Deog-Bon Choo, Young-Kug
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제31권 제1호
발행연도
2008.1
수록면
88 - 95 (8page)

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Gangliosides are a family of sialic acid-containing glycosphingolipids that are abundant in neurons and have a variety of functions in developing and mature tissues. We examined the expression of ganglioside GT1b in the embryonic preimplantation stage after freezing and thawing processes to determine the regulatory roles of ganglioside GT1b in early embryonic development. ICR mouse embryos at the two-cell stage obtained by flushing the oviducts were frozen by two cryopreservation procedures, slow freezing using a programmable freezer or vitrification by direct plunging into liquid nitrogen. Slow freezing was conducted with equilibration in 1.5 M 1,2-propanediol or 5% equilibration glycerol. Vitrification was applied with a 10-15 min equilibration in 7.5% ethylene glycol (EG), 7.5% dimethylsulfoxide (DMSO), and 30 sec in a solution of 15% EG, 15% EMSO and 0.5 M sucrose. Immediately after thawing, the survival rate of the embryos was assessed by their morphology and ability to develop to blastocysts in culture. The survival rate of vitrified and thawed embryos (92%) was significantly higher than that of slow frozen and thawed embryos (76%) (P<0.05). A tendency of higher blastocyst rate was found in the vitrified and thawed embryos compared to that of the slow frozen and thawed embryos. Confocal immunofluorescence staining confirmed that surviving embryos expressed ganglioside GT1b, with the strongest expression at the compacted eight-cell or later stage embryos. Ganglioside GT1b was not observed in the TUNEL-positive, apoptotic embryos, suggesting that cryopreservation had induced DNA breaks in them. These results suggest that ganglioside GT1b may play an important role in embryo survival or development.
#Slow freezing #Vitrification #Ganglioside GT1b

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