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논문 기본 정보

자료유형
학술저널
저자정보
Xin, Jiaying (Key Laboratory for Food Science & Engineering, Harbin University of Commerce) Lin, Kai (Key Laboratory for Food Science & Engineering, Harbin University of Commerce) Wang, Yan (Key Laboratory for Food Science & Engineering, Harbin University of Commerce) Xia, Chungu (State Key Laboratory for Oxo Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제58권 제3호
발행연도
2015.1
수록면
387 - 395 (9page)

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The present study reported a novel approach for the green synthesis of gold nanoparticles (AuNPs) by using methanobactin (Mb) from the spent medium of Methylosinus trichosporium 3011. This paper explored the synthesis of AuNPs under different Mb concentrations, temperatures, and reaction times. The biosynthesized AuNPs were characterized by using UV-Visible spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). Comparing with the previous research using hydroquinone as an extra electron donor to synthesis of AuNPs, this research was the first to show that rising temperature improved the formation rate of AuNPs in a higher concentration of Mb itself. The formation of AuNPs was confirmed by surface plasmon resonance using UV-Visible spectrophotometry. Fluorescence spectroscopy experiments revealed that Mb sample showed three prominent peaks. In addition, the fluorescence exhibited by Mb was rapidly quenched upon addition of $HAuCl_4$. According to the image analysis performed on a TEM micrograph, the obtained AuNPs were predominantly spherical with an average size of $19.3{\pm}5.5nm$. XPS analysis demonstrated that Mb molecules reduced Au(III) to Au(0). FTIR spectrum confirmed the involvement of biomass groups in capping and reduction of AuNPs, which increased the stability of the synthesized AuNPs.

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