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Bacterial Reverse Mutation Test of CP pharmacopunture
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세균을 이용한 CP 약침의 복귀돌연변이 시험

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Type
Academic journal
Author
Ji Hye Hwang (가천대학교) Jaseung Ku (보광한의원) Chul Jung (남상천한의원)
Journal
Korean Oriental Medical Society The Journal of Korean Medicine Vol.41 No.3 KCI Accredited Journals
Published
2020.9
Pages
55 - 65 (11page)

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Bacterial Reverse Mutation Test of CP pharmacopunture
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Objectives: This study aimed to evaluate the toxicity of CP pharmacopunture using bacterial reverse mutation test.
Methods: To determine the mutagenic potential of CP pharmacopunture, histidine requiring Salmonella typhimurium (TA98, TA100, TA1535, and TA1537) and tryptophan requiring Escherichia coli (WP2uvrA, pKM101) strains were used. The negative (normal saline solution) and positive (Sodium azide, 2-Nitrofluorene, 2-Aminoanthracene, 9-Aminoacridine, and 4-Nitroquinoline N-oxide) control groups were used. To determine the dose levels of the main study, a dose range-finding study was conducted.
Results: As a results of the dose range-finding study, the growth inhibition by CP pharmacopunture was not evident at any dose levels in the absence and presence of metabolic activation. As a results of the main study, the mean number of revertant colonies was less than twice when compared to the negative control values at all dose levels of the CP pharmacopuncture in the presence and absence of metabolic activation, showing no dose-related increase. In the positive control group, the number of revertant colonies was markedly increased by more than twice when compared to the negative control group.
Conclusion: According to the results of this study, CP pharmacopunture did not show any signs of mutagenic potential.
#capsaicin #CP pharmacopunture #toxicity #bacterial reverse mutation test #acupuncture

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References (32)

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Jung C / 2011 / A clinical study of immune pharmacopuncturology / Kyungrak medical publishing co :127~133 google schola Nam SC / 2009 / Immune Pharmacopuncturology / Kyungrak medical publishing co google schola 2020 / Korean Acupuncture & Moxibustion Society / Hanmi Medical Publishing Co google schola Ramírez-Barrantes R / 2018 / Transient receptor potential vanilloid 1expression mediates capsaicin-induced cell death / Frontiers in physiology 9:682 google schola Tandan R / 1992 / Topical capsaicin in painful diabetic neuropathy : controlled study with long-term follow-up / Diabetes care 15(1):8~14 google schola

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