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논문 기본 정보

자료유형
학술저널
저자정보
Choi, Seung-Hee (College of Pharmacy, Seoul National University) Kim, Byong-Kak (College of Pharmacy, Seoul National University) Kim, Ha-Won (College of Pharmacy, Seoul National University) Kwak, Jin-Hwan (College of Pharmacy, Seoul National University) Park, Eung-Chil (College of Pharmacy, Seoul National University) Kim, Young-Choong (College of Pharmacy, Seoul National University) Yoo, Young-Bok (Institute of Agricultural Sciences) Park, Yong-Hwan (Institute of Agricultural Sciences)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제10권 제3호
발행연도
1987.1
수록면
158 - 164 (7page)

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To obtain a new strain of Ganoderma lucidum by protoplast fusion technique, its protoplast formation and regeneration were studied. Several factors affecting the protoplast formation and regeneration were investigated to find their optimum conditions. The mycelium was grown for four days on the cellophane membrane placed on G. Incidum complete medium (GCM). When various commercial lytic enzymes were examined for protoplast isolation, the combination of Novozym 234 and $\beta$glucuronidase was found to be effective. An osmotic stabilizer, 0.6 M sucrose in 20 mM phosphate buffer pH 5.8, gave the highest yield of protoplasts. Three-hour incubation in shaking incubator was most suitable for releasing protoplasts. To increase the protoplast yield, pretreatment with 2-mercaptoethanol was carried out. The regeneration frequency in GCM containing 0.6M MgSO$_4$ 7$H_2O$ was shown to be 0.66%.

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