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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Chul-Sang (Department of Biological Science, Kunsan National University) Lee, Doo-Soo (Hanmi Research Center) Fang, Nan-Zhu (Korea Research Institute of Bioscience and Biotechnology) Oh, Keon-Bong (Korea Research Institute of Bioscience and Biotechnology) Shin, Sang-Tae (College of Veterinary Medicine, Chungnam National University) Lee, Kyung-Kwang (Korea Research Institute of Bioscience and Biotechnology)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제30권 제4호
발행연도
2006.1
수록면
293 - 299 (7page)

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In order to generate transgenic goats expressing human growth hormone (hGH) in their mammary glands, goat ${\beta}-Casein/hGH$ hybrid gene was introduced into goat zygotes by pronuclear microinjection. DNA-injected embryos were transferred to the oviduct of recipients at 2-cell stage or to the uterus at morula/blastocyst stage after cultivation in glutathione-supplemented mSOF medium in vitro. Pregnancy and survival rate were not significantly different between 2-cell embryos and morula/blastocysts transferred to oviduct and uterus, respectively. One transgenic female goat was generated from 153 embryos survived from DNA injection. Southern blot analysis revealed that the transgenic goat harbored single-copy transgene with a partial deletion in its sequences. Despite of the partial sequence deletion, the transgene was successfully expressed hGH at the level of $72.1{\pm}15.1{\mu}g/ml$ in milk throughout lactation period, suggesting that the sequence deletion had occurred in non-essential part of the transgene for the transgene expression. Unfortunately, however, the transgene was not transmitted to her offspring during three successive breeding seasons. These results demonstrated that goat ${\beta}-casein/hGH$ gene was integrated into the transgenic goat genome in a mosaic fashion with a partial sequence deletion, which could result in a low level expression of hGH and a failure of transgene transmission.

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