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논문 기본 정보

자료유형
학술저널
저자정보
Chae, Soon-Yong (Korea Ginseng and Tobacco Research Institute) Park, Eun-Kyung (Korea Ginseng and Tobacco Research Institute) Kim, Young-Ho (Korea Ginseng and Tobacco Research Institute) Kim, Sang-Seock (Korea Ginseng and Tobacco Research Institute) Paek, Kyung-Hee (Graduate School of Biotechnology, Korea University)
저널정보
한국연초학회 한국연초학회지 한국연초학회지 제20권 제1호
발행연도
1998.1
수록면
57 - 65 (9page)

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초록· 키워드

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This study was conducted to develop a resistant tobarro against Potato virus Y (PVY) by transformation of the plants with genetically engineered viral genes. The complimentary DNAs (cDNAS) of potato virus Y-necrosis strain (PVY-Vn) replicase mutant genes (3'-deleted, 5'-deleted and ADD-mutant Nlbs) were synthesized through RT-PCR by using purified PVY-VN RNA and synthesized primers, and cloned in the sense orientation into a plant expression vector (pMBPI), The cDNAS of the genes were transferred into Agrobacterium tumefaciens LBA 4404, and then transformed into tobacco (Nicotiana tabacum cv. Burley 21) plants. Regenerated plants were tested for PVY resistance by inoculation test; 13 transgenic plants including 7 for 3'-deleted Nlb, 3 for 5'-deleted Nlb, and 3 for ADD-mutant Nlb appeared to be resistant at 4 weeks after inoculation with PVY-VN. Among the 13 transgenic tobacco plants, 8 plants had no symptom up to 14 weeks after inoculation. The progenies ($T_1$) from self-fertilization of the transgenic lines varied 0.0% to 81.2% in their resistance (% of resistant plants). The analysis of Nlb-31deleted, -5'deleted and -ADD mutant in the $T_1$ plants by polymerase chain reaction (PCR) showed that Nlb-3'deleted, -5'deleted and -ADD mutants were detected in all of the resistant plants. These results suggest that the PVY resistance was inherited in the $T_1$ generation.

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