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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Although efficient shoot regeneration and selection are essential for genetic transformation mediated by Agrobacterium, success has been limited with the garland chrysanthemum (Chrysanthemum coronarium L.). In this study, we developed a useful protocol for shoot regeneration with leaf disk explants. The optimal concentrations of NM and BA were 0.2 mg $L^{-1}$ and 0.5 mg $L^{-1}$, respectively. To optimize the selection system for regenerating plants from genetically transformed tissues, we tested the effects of four antibiotics (kanamycin, hygromycin, carbenicillin, and cefotaxime). Among them, 5mg $L^{-1}$ hygromycin proved adequate as a selectable marker, whereas 500mg $L^{-1}$ carbenicillin was effective in eliminating excessive Agrobacterium after co-cultivation. Transgenic plants were obtained by first co-culturing garland chrysanthemum leaf disks with A. tumefaciens strain EHA105, which harbors plasmid pRCVII containing the hygromycin resistance (hpt) and $\beta-glucuronidase$ (GUS) genes. After the transgenic plants were confirmed via Southern analysis, they were rooted in soil and appeared phenotypically normal. Our report is the first to describe the optimum conditions for producing transgenic plants of this species.
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