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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Sang-Sun (Major in Biological Science and Education, Graduate School, Korea National University of Education) Hong, Sung-Woon (Major in Biological Science and Education, Graduate School, Korea National University of Education) Kim, Seung-Hae (Division of Fungal Research, Bioneer Corp. Ltd) Kim, Bong-Cheol (Division of Fungal Research, Bioneer Corp. Ltd)
저널정보
한국균학회 Mycobiology Mycobiology 제29권 제3호
발행연도
2001.1
수록면
135 - 141 (7page)

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Differential display of reverse transcription(DDRT)-PCR was conducted to have a profile of the differentially expressed genes during the formation of fruiting body of Lentinus edodes. The lines of L. edodes(ImHyup-1) employed were cultivated in the artificial blocks of sawdust, and the fruiting body was induced from the mycelia or the mass protruded from the brown surface of the sawdust blocks. RNAs were prepared from the four different developmental stages; mycelial, primordial, and stipes and pileus of fruiting body. The fragments of cDNA were synthesized from the combinations of the arbitrary primers and 3' one anchored Oligo-dT primer. Twelve combinations using the primers have been tested, and among them nineteen bands were identified as differentially expressed. Those genes were further analyzed by DNA sequencing and followed by homology search. Characterization of one clone was conducted as a preliminary data and more are under investigation.

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