CHEMILUMINESCENCE STUDIES ON THE BIOLOGICAl, INTERACTION BETWEEN SUPEROXIDE ANION RADICAL AND NITRIC OXIDE PRODUCED BY PHORBOI, ESTER-STIMULATED RAW264.7 MACROPHAGES

Lee, Hong; Pae, Hyun-Ock; Jun, Chang-Duk; Kwak, Hyun-Jeong; Park, Rae-Kil; Yoo, Ji-Chang; Lee, Seog-Jae; Kim, Myung-Sun; Kim, Hae-Song; Choi, Byung-Min; Chung, Hun-Taeg

추천

검색

자료유형: 학술저널

저자정보: Lee, Hong (Department of Chemistry, Wonkwang University) Pae, Hyun-Ock (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) Jun, Chang-Duk (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) Kwak, Hyun-Jeong (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) Park, Rae-Kil (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) Yoo, Ji-Chang (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) Lee, Seog-Jae (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) Kim, Myung-Sun (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) Kim, Hae-Song (Department of Chemistry, Wonkwang University, Department of Microbiology and lmmunology, Wonkwang University) Choi, Byung-Min (Department of Chemistry, Wonkwang University, Department of Micro) Chung, Hun-Taeg

저널정보: 한국광과학회 Journal of photoscience : an international journal officail organ of the korean society of photoscience Journal of photoscience : an international journal officail organ of the korean society of photoscience 제4권 제2호

발행연도: 1997.1

수록면: 31 - 34 (4page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

The rapid and spontaneous interaction between superoxide anion radical and nitric oxide to yield the potent oxidants. peroxynitrite artion and peroxynitrous acid, was investigated in phorbol myristate acetate(PMA)-stimulated RAW264.7 macrophases by means of lucigenin- or luminol-enhanced chemiluminescence method. When RAW264.7 macrophages were stimulated by PMA. peroxynitrite-induced chemiluminescence was clearly observed. To prove observed chemiluminescencc due to the reaction between superoxide anion radical and nitric oxide produced by RAW264.7 macrophases, N-nitrosoglutathione (GSNO), a nitric oxide-releasing compound. superoxide dismutase(SOD), an enzyme removing superoxide anion radical by dismutating superoxide artion radical to hydrogen peroxide, and N-acethyl cysteine(NAC), a scarvenging reagent both superoxide artion radical and nitric oxide, were added in the cell system. Peroxynitrite- induced chemilumincscence was increased by exogenous addition of GSNO. whereas observed chemiluminescence was decreased by SOD and NAC. These results suggest that PMA-stimulated RAW264.7 macrophages produce both superoxide anion radical and nitric oxide to form peroxynitrite.

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

Lee, Hong

소속기관 Department of Chemistry, Wonkwang University

주요연구분야 자연과학 > 물리학

논문수 1 이용수 1

Pae, Hyun-Ock