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Previously, a chloroplast-localized Cu,Zn superoxide dismutase (chCu,ZnSOD) was isolated from lily and the sense- and antisense- sequences of the lily chCu,ZnSOD were used to transform potato plants. Two selected lines, the sense- and anti-sense strand of transgenic plants, were further characterized for resistance to Erwinia carotovora, which is a severe pathogen affecting potato plants. Only the sense-strand transgenic potato, which contained less O₂<SUP>.?</SUP> and more H₂O₂ than wild-type and antisense-strand transgenic plants, showed increased resistance to E. carotovora. Additional studies using O₂<SUP>.?</SUP> or H₂O₂ scavengers in wild-type, sense-strand, and antisense-strand transgenic plants suggest that resistance to E. carotovora is induced by reduced O₂<SUP>.?</SUP> and is not influenced by H₂O₂. To the best of our knowledge, this report is the first study suggesting that resistance to E. carotovora is enhanced by reduced O₂<SUP>.?</SUP>, and not by increased amounts of H₂O₂.

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UCI(KEPA) : I410-ECN-0101-2009-481-014772270