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논문 기본 정보

자료유형
학술저널
저자정보
김선정 (유전공학연구소 생물자원그룹) 이고운 (유전공학연구소 생물자원그룹) 배수경 (부산대학교 분자생물학과) 조용연 (국립보건안전연구원) 한용만 (유전공학연구소 생물자원그룹) 이철상 (유전공학연구소 생물자원그룹) 이경광 (유전공학연구소 생물자원그룹) 유대열 (유전공학연구소 생물자원그룹)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제18권 제2호
발행연도
1994.1
수록면
133 - 139 (7page)

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Two human lactoferrin expression vectors(pCChcLf and pCChcLf-1) were constructed using rat $\beta$-casein gene and human lactoferrin cDNA. The recombinant DNAs containing human lactoferrin cDNA were microinjected into the fertilized eggs of hybrid mice (BDF1 : C57BL$\times$DBA) and the DNA-injected eggs were treansferred into the oviducts of foster mothers. Genomic DNAs were isolated from the tails of mice born from the microinjected eggs and analyzed by Southern blot analysis. As a result, 5 and 9 transgenic mice with CChcLf and CChcLf-1 gene were produced, respectively. To determine tissue-specificity of transgene expression, Northern blot analysis was performed. Female transgenic mice were killed at day 10 of lactation and total RNAs from various tissues were isolated. Based on Northern blot analysis, it was shown that transgene was mainly expressed in the mammary glands of transgenic mice. In addition, the human lactoferrin in milk was detected by enzyme-linked immunosorbent assay. For this study, milk was obtained from the mammary glands of the transgenic mice at day 10 of lactation. In line #2 of CChcLf and line #7 of CChcLf-1 transgenic mice, human lactoferrin was secreted into the milk at concentration levels of 340ng/ml and 60ng/ml, respectively.

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