지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 556 - 561 (6page)
이용수
초록· 키워드
오류제보하기
Lactoferrin is an 80-kDa iron-binding glycoprotein that is found in high concentrations in human milk. Human lactoferrin (hLF) has several beneficial biological activities including immune system modulation and antimicrobial activity. In the present study, we devolved a method of hLF expression through introducing the hLF gene construct into Oriza sativa cv. Nakdong using the Agrobacterium-mediated transformation system. The expression of the hLF gene under the control of the rice glutelin promoter was detected in the seeds of transgenic rice plants. Transformed rice plants were selected on media containing herbicide(DL-phosphinothricin) and the integration of hLF cDNA was confirmed by Southern blot analysis. The expression of the full length hLF protein from the grains of transgenic rice plants was verified by Western blot analysis. The lactoferrin expression levels in the transformed rice grains determined by enzyme-linked immunosorbant assay accounted for approximately 1.5% of total soluble protein. Taken together, these data indicate that rice grains expressing hLF can be directly incorporated into infant formula and baby food.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Arakawa T / 1999 / Improvements in human health through production of human milk proteins in transgenic food plants. in: Chemicals via Higher Plant Bioengineering / Kluwer Academic/Plenu
Bradford M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 24
Choi SM / 2003 / High expression of a human lactoferrin in transgenic tobacco cell cultures / Biotech Lett 25 : 213 ~ 218
Chong DKX / 1997 / Expression of the human milk protein β-casein in transgenic potato plants / Transgenic Res 6 : 289 ~ 296
Chong DKX / 2000 / Expression of full-length bioactive antimicrobial human lactoferrin in potato plants / Transgenic Res 9 : 71 ~ 78
Bradford M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72 : 24
Choi SM / 2003 / High expression of a human lactoferrin in transgenic tobacco cell cultures / Biotech Lett 25 : 213 ~ 218
Chong DKX / 1997 / Expression of the human milk protein β-casein in transgenic potato plants / Transgenic Res 6 : 289 ~ 296
Chong DKX / 2000 / Expression of full-length bioactive antimicrobial human lactoferrin in potato plants / Transgenic Res 9 : 71 ~ 78
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Expression of Human Lactoferrin Gene in Transgenic Rice(Oryza sativa L.)
Journal of Plant Biotechnology
2007 .01
-
Expression and Characterization of the Human Lactoferrin in the Milk of Transgenic Mice
한국수정란이식학회 심포지움
2003 .01
-
인체 락토페린 생산 형질전환 가시오갈피 배양세포
Journal of Plant Biotechnology
2005 .01
-
Pichia pastoris에서 Human Lactoferrin의 발현
한국식품영양과학회지
1997 .08
-
Co-expression of Human Proteins (IL-10, TPO and/or Lactoferrin) into Milk of Cross-Breed Transgenic Mouse
한국동물번식학회지
2008 .01
-
인체 락토페린 생산 형질전환 고구마 개발
Journal of Plant Biotechnology
2009 .01
-
흰쥐 베타-카제인 유전자의 발현조절 부위를 이용하여 유선에서 사람 락토페린을 발현하는 형질전환 생쥐의 개발
한국동물번식학회지
1994 .01
-
Transmission of Bovine $\beta-Casein/Human$ Lactoferrin Fusion Gene in Transgenic Cattle
한국동물번식학회지
2005 .01
-
Expression of Antimicrobial Gene, Asa-AMP in Transgenic Rice
한국토양비료학회 학술발표회 초록집
2000 .05
-
한우 Lactoferrin 가수분해물의 항균 활성
한국축산식품학회지
1998 .01
-
Rice Histone Deacetylase : Characterization and Expression in Transgenic Rice Plants
한국토양비료학회 학술발표회 초록집
2000 .05
-
PMA로 자극되어진 세포에서 염증 Cytokine 발현에 미치는 Bovine Lactoferrin의 생물활성 영향
한국축산식품학회지
2012 .01
-
Transgenic Rice Plants Expressing Rice Histone Deacetylase
한국토양비료학회 학술발표회 초록집
2001 .05
-
Overexpression of starch branching enzyme 1 gene improves eating quality in japonica rice
Journal of Plant Biotechnology
2013 .01
-
제초제 저항성 형질전환 벼 계통의 농업적 특성과 분자생물학적 평가
한국육종학회지
2007 .01
-
Lactoferrin 유화액의 안정도 특성
한국식품영양과학회 학술대회발표집
2012 .10
-
연구단보(Research Note) : Phospholipid Liposomes과 Bovine Lactoferrin의 상호작용
Korean Journal of Agricultural Science
2010 .12
-
한우 Lactoferrin이 T cell의 활성화에 미치는 효과
한국축산식품학회 학술발표초록집
2001 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0