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논문 기본 정보

자료유형
학술저널
저자정보
김지영 (아주대학교 생물공학과) 김공환 (아주대학교 생물공학과) 김경자 (순천향대학교 유전공학과) 구양모 (서울대학교 약학과)
저널정보
대한약학회 약학회지 약학회지 제38권 제5호
발행연도
1994.1
수록면
614 - 620 (7page)

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초록· 키워드

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Following the study on the fermentation conditions influencing the production of vitamin $B_{12}$ by Propionibacterium shermanii(Korean J Biotechnol. Bioeng. 7,126-131, 1992), the effects of some factors supplemented in the medium on the production of vitamin $B_{12}$ were studied. Maximum production of vitamin $B_{12}$ was observed when $Co^{+2}$ was supplemented at the concentration of 2-4 ppm in the fermentation medium. Increase of the supplemented $Co^{+2}$ to 12 ppm did not inhibit the growth of the organism, but it accelerated the lysis of the organism. In the literature, peptone was reported to activate the biosynthesis of vitamin $B_{12}$. Examination of the effect of peptone on the growth and the production of vitamin $B_{12}$ showed that at early stage more vitamin$B_{12}$ was observed in the supplemented medium, but no difference was observed in the later stage of fermentation. Examination of the time for addition and the amount of 5,6-dimethylbenzimidazole, a precursor known to influence the production of vitamin $B_{12}$, showed that a maximum yield of vitamin $B_{12}$ was observed when 15 mg/L was added to the fermentation medium after 2 days' incubation. The effect was comparable with the increase of the production of vitamin $B_{12}$ when the fermentation condition was changed to aerobic condition after 2 days' culture under anaerobic condition.

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