백서의 가역성 및 비가역성 뇌허혈 모형의 실험적 고찰

송광철; 최병연; 김성호; 배장호; 김오룡; 조수호

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자료유형: 학술저널

저자정보: 송광철 (영남대학교 의과대학 신경외과학교실) 최병연 (영남대학교 의과대학 신경외과학교실) 김성호 (영남대학교 의과대학 신경외과학교실) 배장호 (영남대학교 의과대학 신경외과학교실) 김오룡 (영남대학교 의과대학 신경외과학교실) 조수호 (영남대학교 의과대학 신경외과학교실)

저널정보: 대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제29권 제7호

발행연도: 2000.1

수록면: 853 - 860 (8page)

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Objective : The purpose of our experimental study was to analysis the advantages and disadvantages in the reversible and irreversible cerebral ischemic models with rats by staining with Neutral Red(NR) solusion, 2% 2,3,5-triphenyltetrazolium chloride(TTC) and Hematoxylin & Eosin(H & E). Methods : We have measured the range of cerebral infarction in the rat to get a suitable ischemic model along the object of study with and without craniectomy. With craniectomy, 9 rats were sacrificed for irreversible cerebral ischemic model by means of ligation at proximal(group I) and distal(group II), and coagulation at proximal(group III) middle cerebral artery. Also, 6 rats were sacrificed for irreversible(group IV) and reversible(group V) cerebral ischemic model using nylon thread without craniectomy. The sizes of infarction were measured by staining the coronal sections of the brain with NR solusion, TTC and H & E. Results : There are no difference of physiological parameters comparing the each group. Cerebral infarction was not observed in group II, but it's volume was largest in group IV. Disadvantages of craniectomy group(I, II, III) are the long duration of operation and cortical damage by procedure. It's advantage is confirmation of the middle cerebral artery occlusion and cessation of blood flow through the operative microscope. In case of ischemic models using nylon thread (group IV, V), it is hard to identify the interruption or recirculation of blood flow through the middle cerebral artery, but the advantage is the simplicity of operative technique which reduces the operation time and minimizes the cerebral damage due to craniectomy. Therefore, it seems important to set up the reversible and irreversible ischemic models by carefully considering advantages and disadvantages listed above. Conclusion : TTC staining seems to be effective since it reflects the histological damage sufficiently and quickly. It is hoped that researches focused on ischemic penumbra, which became popular recently, will be further carried on with use of NR staining, optical microscope and electron microscope.

#Cerebral ischemic model #TTC #NR #H & #amp #amp # E

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