ISOLATION OF A NEW $alpha$-GLUCOSIDASE INHIBITOR FROM A FUNGUS, PENICILLIUM SP. F70614

Kwon, Oh-Sung; Park, Sang-Ho; Lee, Sang-Hwa; Park, Dong-Jin; Yun, Bong-Sik; Kim, Chang-Jin

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자료유형: 학술대회자료

저자정보: Kwon, Oh-Sung (Korea Research Institute of Bioscience and Biotechnology) Park, Sang-Ho (Korea Research Institute of Bioscience and Biotechnology) Lee, Sang-Hwa (Korea Research Institute of Bioscience and Biotechnology) Park, Dong-Jin (Korea Research Institute of Bioscience and Biotechnology) Yun, Bong-Sik (Korea Research Institute of Bioscience and Biotechnology) Kim, Chang-Jin (Korea Research Institute of Bioscience and Biotechnology)

저널정보: 한국응용약물학회 한국응용약물학회 추계학술발표논문집 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products

발행연도: 1998.1

수록면: 134 - 134 (1page)

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The modulation of glycosidase activity by inhibitors is of great interest. Such compounds have been shown to be important tools in mechanistic studies on glycohydrolase as well as having promising therapeutic application. An ${\alpha}$-glucosidase inhibitor was isolated from culture filterates of Penicillium sp. The inhibitor was active against ${\alpha}$-glucosidase isolated from yeast and porcine small intestine. However, it showed no inhibition to Aspergillus ${\alpha}$-galactosidase, Escherichia coli ${\beta}$-galactosidase, and jack bean ${\alpha}$-mannosidase. The inhibitor was highly soluble in ether, methanol and chloroform. The inhibitor was purified using silica gel, Sephadex LH-20 column chromatography and reverse-phase HPLC. The inhibitory compound designated PA-7(IC$\sub$50/=35$\mu\textrm{g}$) was obtained as white powder. The structure of PA-7 was determined with spectroscopic data of EI-MS, FAB-MS, $^1$H, and $\^$13/C NMR. The inhibitor has a diketopiperazine moiety.

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