Transepithelial Transport of Organic Cation and Its Inhibition by Sulfhydryl and Carboxyl Reagents in Opossum Kidney Cell Monolayer

Woo, Jae-Suk; Oh, Se-Ok; Jung, Jin-Sup; Kim, Yong-Keun; Lee, Sang-Ho

추천

검색

자료유형: 학술저널

저자정보: Woo, Jae-Suk (Department of Physiology, College of Medicine, Pusan National University) Oh, Se-Ok (Department of Physiology, College of Medicine, Pusan National University) Jung, Jin-Sup (Department of Physiology, College of Medicine, Pusan National University) Kim, Yong-Keun (Department of Physiology, College of Medicine, Pusan National University) Lee, Sang-Ho (Department of Physiology, College of Medicine, Pusan National University)

저널정보: 대한생리학회 대한생리학회지 대한생리학회지 제30권 제1호

발행연도: 1996.1

수록면: 53 - 62 (10page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Transepithelial transport of tetraethylammonium (TEA) was studied in monolayers of opossum kidney cells cultured on permeable membrane filters. $[^{14}C]-TEA$ was transported across the OK cell monolayer from basolateral to apical side by a saturable process which can be stimulated by acidification of the apical medium. The apparent Michaelis-Menten constant $(K_{m})$ and the maximum velocity$(V_{max})$ for the transport were $41\;{\mu}M$ and 147 pmole/ mg protein/ min, respectively. The transport was significantly inhibited by unlabelled TEA, amiloride, cimetidine, choline, and mepiperphenidol added to the basolateral side at 1 mM and was slightly inhibited by 5 mM $N_{1}-methylnicotinamide\;(NMN).$ Unlabelled TEA added to the apical side stimulated the $basolateral-to-apical\;{^{14}C}-TEA$ transport, suggesting that the TEA self-exchange mechanism was involved at the apical membrane. Sulfhydryl reagents such as ${\rho}-chloromercuribenzoic\;acid\;(PCMB)\;and \;{\rho}-chloro-mercuribenzene\;sulfonate \;(PCMBS)$ and carboxyl reagents such as N,N'-dicyclohexylcarbodiimidem (DCCD) and N-ethoxy-carbonyl-2-ethoxy-1,2-dihydro-quinoline(EEDQ) inhibited the TEA transport at both the basolateral and apical membranes of the OK cell monolayer. These results suggest that OK cell monolayers possess a vectorial transport system for organic cations which is similar to that for organic cation secretion in the renal proximal tubule.

#Tetraethylammonium transport #Organic cation #Sulfhydryl reagent #Carboxyl reagent

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!