이화명충(Chilo suppressalis Walker)의 변태에 따른 총단백질, 비단백성실소, 혈단백질, 트리하로오스 지질, 무기염류의 분석 결과와 호흡능, 호흡효소, 효소의 활성을 측정하여 형태적 변화와 비교한 결과는 다음과 같다. 1) 총단백질함량은 용 2일까지 감소하다가 용 4일에서 증가하였고, 종령유충과 성충 2일에서 가장 높게 나타났다. 비단백성실소는 성충 2일에서 가장 높은 농도를 나타냈다. 2) 혈단백질함량은 종령유충과 용 4일에서 가장 높았다. 혈단백질 패턴의 밴드는 종령유충에서 14, 전용에서 7, 용 4일에 9, 용 10일에 9, 우화성충에서 11개가 분이되 변화를 나타냈다. 3) 트리하로오스의 함량은 용 4일에서 낮았고 용화하면서 감소하다가 용기말에서 증가하였다. 4) 지질은 린지질, 모노ㆍ디ㆍ트리아실글리세롤, 콜레스테롤이 분이되었고 트리아실글리세롤이 가장 높아 변태기에 따라 U자형 생리곡선을 나타났다. 5) 호흡능의 변화는 대소양, 숙신산은 성충에서 가장 높았고 용 2일에서 감소하다가 용기말에서 다시 증가하여 숙신산 수소이탈효소의 활성과 비슷한 U자형 생리곡선을 나타냈다. 6) phosphatase의 활성은 acid phosphatase는 용 2일까지 증가하다가 용 4일에서 감소하고 용기말과 우화성충에서 증가하였다. alkaline phosphatase의 활성은 용화하면서 성충에 이르기까지 활성이 감소하였다. transaminase(GOT, GPT)의 활성은 변태에 따라 U자형 생리곡선을 나타냈다. 7) 무기염류의 함량 변화는 용기말에서 증가하여 성충에서 가장 높게 나타났다. 이상의 결과로 혈림프의 성분과 함량의 변화 그리고 효소의 활성변화는 변태에 따라 생리곡선인 U자형 변화를 나타내어 유충기관의 해소와 성충기관의 신생인 형태적 변화와 일치한다. 이는 생리적 쇠약기와 해충방제의 최적기를 구명하는 기초자료가 된다.
The concentration of total protein and non-protein nitrogen, whole body protein patterns, trehalose, lipids and the activities of acid. alkaline phosphatase, glutamic pyruvic transaminase, glutamic oxaloacetic transaminase and succinic acid dehydrogenase and mineral salts during the metamorphosis of the rice stem borer, Chilo suppressalis Walkerwere measured using micro-Kjeldahl method, polyacrylamide gel electrophoresis method, gas chromatographic method, thin layer chromatographic method, Warburg's manometric method, King's method, Reitman's method and Thunberg's method, Schneider methods, respectively. Healthy specimens were choson as samples of each developmental stages: the final instar larva, the newly molted pupa, pupa at 2, 4, 6, 8, and 10 days, newly emerged adult and adults. The total protein concentration decreased gradually until the two day pupa and increased at four day pupa. In the final instar larval stage, the newly emerged adult and two day adult stage the concentration reached a maximum. The non-protein nitrogen concentration increased gradually until the newly netted pupa and decreased at the two day pupa. The protein bands were numbered 1-14 according to their mobilities. A total of 14 protein patterns were detectable on the electrophorograms of whole body homogenates. Each developmental stage qualitative differences in the protein bands. In general, the protein bands were increased with the growth of larva, decreased at newly molted pupal stage, and at pupal and adults stages, were increased gradually, again. The hemolymph trehalose concentration decreased gradually until the four day pupa and increased at the six day pupa. In the last instar larval stage and the newly emerged adult stage the concentration reached a maximum. Phospholipid, monoacylglycerol, diacylglycerol, triacylglycerol, free cholesterol and ester cholesterol were identified at each statges. Triacyglycerol at the final instar larva the concentration reached maximum. Triacylglycerol decreased gradually until prepupa, two days pupa, four and six days pupa and increased at eight days pupal stage. Endogenous respiration was very high at the two day pupal stage, decreased at the six day pupa, and was followed by an increase at the late pupal stages. Succinate in the respiratory substrate increased respiratory activity in all stages and had a'striking influence on both the late pupal stage and the adult. The activities of acid phosphatase generally increased until the two day pupa and decreased at the four day Pupal stage and at eight day pupa and newly emerged adult stages they were increased again. The activity of alkaline phosphatase is by far lower 1.han that of acid phosphatase. And in the throughout all stages there no significant differences between both control and treatments group of alkaline phosphatase. The activities of glutamic pyruvic transaminase and glutamic oxaloacetic transaminase decreased gradually from final instar larva until the ten day pupal stage and increased at newly emerged adult stage. The activity of succinic acid dehydrogenase decreased gradually until the six day pupa and increased at eight day pupal stage. In the final instar larva stage and the two day adult stage the activities reached a maximum. The mineral salts present are calcium, phosphorus, potassium, sodium and lithium identified at each stages. Hence, changes in total protein concentration, protein patterns, trehalose, lipids, mineral salts, acid-alkaline phosphatase activity, activities of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase and succinic acid dehydrogenase through the course of the physiological cycle form a U-shaped curve when graphed against the histolysis of larval organs and the histogensis of adult organs. From a control point of view, the U-shaped pattern of physiological activity indicates that control will be most effective at the time of physiological weakness during the four day pupal stage.