솔잎혹파리(Thecodiplosis japonensis Uchi. et Inouye)의 ??속 幼?과 ??을 脫出하여 落下한 土中의 幼?을 對象으로 體液을 分析, 測定코져 종이 크로마토그라피法, micro-Kjeldahl法, 薄層 크로마토그라피法, 왈부르그 檢壓計法. Bessey-Lowry法과 Reitman-Fran-kel法으로 測定 또는 分析하였다. ??속 幼?과 土中의 幼?에 따라 遊離 아미노酸, 總 窒素含量의 變化, 炭水化物의 變化, 脂質의 種類와 變化, 呼吸能, phosphatase, GOT, GPT의 活性度가 測定되었다. 遊離 아미노酸, 總 窒素含量, 脂質含量, 呼吸能, acid phosphatase의 活性, GOT, GPT의 活性은 ??속의 幼?에서 土中의 幼?으로 進行함에 따라 減少하는 傾向을 나타냈다. 그러나 trehalose의 含量과 alkaline phosphatase의 活性은 增加하는 傾向을 나타냈다.
The concentration of ammo acids, total nitrogen, trehalose, lipids and the activities of respiratory, acidㆍalkaline phosphatase, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase during larval stage in pine leaf gall midge, Thecodiplosis japonensis Uchi. et Inouye were measured using Paper chromatographic method, micro-Kjeldahl method, Thin layer chromatographic method, Warburg’s manometric method, Bessey-Lowry method and Reitman-Frankel method, respectively. Healthy specimens were chosen as samples of each larval stages; larva in gall and larva in soil. Amino acids present in the alcoholic extracts were alanine, glutamic acid, glycine, histidine, methionine, proline, threonine, tryptophan and valine. The total nitrogen concentration reached to 31. 348㎎/g during the larva in gall and the larval stage in soil of the value was decreased to 29.027㎎/g. The hemolymph sugar, trehalose value for larva In soil was about two times of the value for larva in gall. Total lipid, phospholipid, monoacylglycerol, triacylglycerol, sterol, free fatty acid and ester cholesterol were identified at larval stages in gall and soil. Triacylglycerol concentration reached high level in contrast with other lipid contents during larvae in gall and larva in soil. Free fatty acid, sterol except decreased lipids during larval stage in soil. Endogenous respiration, succinate of respiratory activities decreased at larval stage in soil compare with larva in gall. The activities of acid phosphatase decreased larval stage In soil but the activities of alkaline phosphatase increased remarkably. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase reached high level of the larva in gall.