A Simple Method for the Detection and Identification of Korean Brown Cattle(Hanwoo) using DHPLC

Kang, Won; Park, Sane-Bum; Nam, Youn-Hyoung; An, Young-Chang; Lee, Sang-Hyun; Park, Jin-Keun; Lee, Jae-Sik; Jang, On-Cheoul; Park, Su-Min; Park, Yong-Hyun; Ko, Kyung-Hee; Moon, Sung-Sil

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자료유형: 학술대회자료

저자정보: Kang, Won (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) Park, Sane-Bum (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) Nam, Youn-Hyoung (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) An, Young-Chang (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) Lee, Sang-Hyun (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) Park, Jin-Keun (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) Lee, Jae-Sik (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) Jang, On-Cheoul (Dept of chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University) Park, Su-Min (Genetbio, Chungnam Animal Science Center) Park, Yong-Hyun (Genetbio, Chungnam Animal Science Center) Ko, Kyung-Hee (Chungnam Animal Science Center, Konyang Univ.) Moon, Sung-Sil (Chungnam Animal Science Ce)

저널정보: 한국축산식품학회 한국축산식품학회 학술발표초록집 한국축산식품학회 2006년도 정기총회 및 제37차 춘계 국제학술발표대회

발행연도: 2006.1

수록면: 179 - 183 (5page)

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Denaturing high-performance liquid chromatography(DHPLC) is used in a wide variety of genetic applications and is an efficient method for detection of mutations involving one or a few nucleotides. We developed a high-throughput DHPLC method for identifying polymorphisms in the MC1R gene that are characteristic of Hanwoo cattle. We compared 10 tissue samples from Hanwoo cattle, 10 samples from Holstein cattle and 10 samples from Hanwoox Holstein crossbred cattle to determine whether DHPLC analysis can be used to distinguish between these genotypes. Samples obtained from Hanwoo cattle had a unique profile of peaks that could be used as a molecular fingerprint for this breed. We also analyzed two hundred samples in a trial in which we were blinded to the genotype of the samples and correctly identified the breed-of-origin of 594 out of 600 sequence variations(99%).

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