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학술저널
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연세대학교 의과대학 Yonsei Medical Journal Yonsei Medical Journal 제60권 제5호
발행연도
2019.1
수록면
461 - 466 (6page)

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Purpose: To investigate the associations between sperm DNA fragmentation (SDF) and embryo formation rate in normal responderwomen to in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI). Materials and Methods: Fifty-three consecutive, fresh IVF/ICSI cycles performed from 2014 to 2017 were selected. All womenwere normal responders (4 to 14 mature oocytes were retrieved) and at least one normally fertilized oocyte with two pronuclei wasobtained in all cycles. Semen was collected on the day of oocyte retrieval, and SDF levels were measured by sperm chromatin dispersiontest (Halosperm assay). At day 3 after insemination, embryo quality was evaluated by morphologic criteria and categorizedas A/B/C/D. Top quality embryo were defined as grade A embryos with seven cells or more. Results: SDF levels showed a positive linear correlation with the male’s age (r=0.307, p=0.025) and a negative linear correlation withsperm motility (r=-0.491, p<0.0001). To achieve top-quality or a grade A embryo formation rate >70%, the cut-off value SDF was<30.7% for each. Among individuals with SDF <30.7%, the median top-quality or grade A embryo formation rate was significantlyhigher than that among individuals with SDF ≥30.7% (38.1% vs. 20.0%, p=0.038; 50% vs. 25.0%, p=0.017). Conclusion: In normal responder women, high SDF level resulted in low day 3 embryo formation rates. Our results suggest a paternaleffect on embryo quality in IVF/ICSI cycles.

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