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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 53 - 61 (9page)
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초록· 키워드
오류제보하기
Cell migration is a central process for recovering from wounds triggered by physical distress besides embryogenesis and cancer metastasis. Wound healing assay is widely used as a fundamental research technique for investigation of two-dimensional cell migration in vitro. The most common approach for imitating physical wound in vitro is mechanical scratching on the surface of the confluent monolayer by using sharp materials. The iron metal pin with a suspension spring for fine adjustment of the orthogonal contact surface between the scratching point and the individual bottom of multi-well plate with planar curvatures were adopted for the creative invention of a 96-well plate wound maker. While classic tips drew diverse and zigzag scratching patterns on the confluent monolayer, our wound maker displayed synchronized linear wounds in the middle of each well of a 96-well plate that was seeded with several cell lines. Given that several types of multi-well plates commercially available are compatible with our lab-made wound maker for creating uniform scratches on the confluent monolayer for the collective cell migration in wound healing assay, it is certain that the application of this wound maker to the real-time wound healing assay in high content screening (HCS) is superior than utilization of typical polypropylene pipette tips.
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등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Chung-Ju Yeh / 2017 / The effects of artocarpin on wound healing: in vitro and in vivo studies / Scientific Reports 7(1):15599
Bainbridge P. / 2013 / Wound healing and the role of fibroblasts / Journal of Wound Care 22(8):407~412
Po Ying Poon / 2017 / A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate / Journal of Visualized Experiments (121)
Daniel Irimia / 2010 / Microfluidic Technologies for Temporal Perturbations of Chemotaxis / Annual Review of Biomedical Engineering 12(1):259~284
Maciej T. Nogalski / 2012 / A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay / Journal of Visualized Experiments (70):E4165
Bainbridge P. / 2013 / Wound healing and the role of fibroblasts / Journal of Wound Care 22(8):407~412
Po Ying Poon / 2017 / A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate / Journal of Visualized Experiments (121)
Daniel Irimia / 2010 / Microfluidic Technologies for Temporal Perturbations of Chemotaxis / Annual Review of Biomedical Engineering 12(1):259~284
Maciej T. Nogalski / 2012 / A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay / Journal of Visualized Experiments (70):E4165
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