지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제5호
- 발행연도
- 2020.1
- 수록면
- 681 - 688 (8page)
이용수
초록· 키워드
오류제보하기
Bacterial cell-based biosensors, or whole-cell bioreporters (WCBs), are an alternative tool for the quantification of hazardous materials. Most WCBs share similar working mechanisms. In brief, the recognition of a target by sensing domains induces a biological event, such as changes in protein conformation or gene expression, providing a basis for quantification. WCBs targeting heavy metal(loid)s employ metalloregulators as sensing domains and control the expression of genes in the presence of target metal(loid) ions, but the diversity of targets, specificity, and sensitivity of these WCBs are limited. In this study, we genetically engineered the metal-binding loop (MBL) of ZntR, which controls the znt-operon in Escherichia coli. In the MBL of ZntR, three Cys sites interact with metal ions. Based on the crystal structure of ZntR, MBL sequences were modified by sitedirected mutagenesis. As a result, the metal-sensing properties of WCBs differed depending on amino acid sequences and the new selectivity to Cr or Pb was observed. Although there is room for improvement, our results support the use of currently available WCBs as a platform to generate new WCBs to target other environmental pollutants including metal(loid)s.
목차
등록된 정보가 없습니다.
참고문헌 (28)
참고문헌 신청
Bereza-Malcolm LT / 2014 / Environmental sensing of heavy metals through whole cell microbial biosensors : a synthetic biology approach / ACS Synthetic Biol 4:535~546
Fernandez-López R / 2015 / Transcription factor-based biosensors enlightened by the analyte / Front. Microbiol 6:648
Mahr R / 2016 / Transcription factor-based biosensors in biotechnology : current state and future prospects / Appl. Microbiol. Bbiotechnol 100:79~90
Robbens J / 2010 / Escherichia coli as a bioreporter in ecotoxicology / Appl. Microbiol. Bbiotechnol 88:1007~1025
윤영대 / 2018 / Modulating the Properties of Metal-Sensing Whole-Cell Bioreporters by Interfering with Escherichia coli Metal Homeostasis / Journal of Microbiology and Biotechnology 28(2):323~329
Fernandez-López R / 2015 / Transcription factor-based biosensors enlightened by the analyte / Front. Microbiol 6:648
Mahr R / 2016 / Transcription factor-based biosensors in biotechnology : current state and future prospects / Appl. Microbiol. Bbiotechnol 100:79~90
Robbens J / 2010 / Escherichia coli as a bioreporter in ecotoxicology / Appl. Microbiol. Bbiotechnol 88:1007~1025
윤영대 / 2018 / Modulating the Properties of Metal-Sensing Whole-Cell Bioreporters by Interfering with Escherichia coli Metal Homeostasis / Journal of Microbiology and Biotechnology 28(2):323~329
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Modulating the Properties of Metal-Sensing Whole-Cell Bioreporters by Interfering with Escherichia coli Metal Homeostasis
Journal of Microbiology and Biotechnology
2018 .01
-
Biochemical and Biodiversity Insights into Heavy Metal Ion-Responsive Transcription Regulators for Synthetic Biological Heavy Metal Sensors
Journal of Microbiology and Biotechnology
2019 .01
-
청보리 생산량의 기후요인 분석을 위한 베이지안 구조방정식 모형
응용통계연구
2016 .02
-
Monitoring of heavy metals contamination in agricultural products at abandoned mine area
한국분석과학회 학술대회
2021 .11
-
Metal artifact reduction of silicon material in head and neck cancer with metal
한국자기학회 학술연구발표회 논문개요집
2019 .11
-
Transmitted Light Characteristics of Sub-skin-depth Metal Arrays
새물리
2023 .05
-
Intrinsic Spin Hall Effect in Ferromagnetic Metal-Heavy Metal Interface
한국자기학회 학술연구발표회 논문개요집
2017 .11
-
Direct domain structure observation depending on relative ratio of metal (FM) and metal-oxide (AFM) in mixture phase film
한국자기학회 학술연구발표회 논문개요집
2017 .05
-
산업단지 유역 하천수와 퇴적물 내 중금속 오염도 평가 및 기원 추적 연구
생태와 환경
2019 .01
-
제주도 자연토양에서 중금속의 농도
한국환경과학회지
2015 .02
-
Short-Term Effects of Low-Level Heavy Metal Contamination on Soil Health Analyzed by Nematode Community Structure
The Plant Pathology Journal
2016 .08
-
Growth and analysis of novel A15 W₃Ta Heavy metal layer
한국자기학회 학술연구발표회 논문개요집
2020 .11
-
The role of metal ions on low energy electron-induced biomolecular alteration
한국진공학회 학술발표회초록집
2017 .02
-
광주지역 황사시 미세먼지 유입경로별 중금속 오염도 평가
한국환경과학회지
2020 .01
-
고속도로 터널내 입자상물질(PM10, PM2.5)의 특성과 중금속의 분석에 관한 연구
한국대기환경학회 학술대회논문집
2017 .11
-
Room temperature ferromagnetism in a magnetic-metal-rich van der Waals metal
한국자기학회 학술연구발표회 논문개요집
2019 .05
-
Microstructure control in transition-metal nitride alloy films via hybrid HIPIMS/magnetron co-sputtering using selective metal-ion irradiation
한국진공학회 학술발표회초록집
2016 .08
-
A Study on the Heavy Metals of Distributed Kimchi in Seoul
한국분석과학회 학술대회
2023 .05
-
Combination of Synthetic Genetic Circuitry and Microfluidics for Highly Sensitive Heavy Metal Ion Biosensors
한국미생물학회 학술대회논문집
2016 .04
-
Heavy metal capping and strain effects on magnetocrystalline anisotropy of 5d transition metal/Fe/MgO : A-first principles study
한국자기학회 학술연구발표회 논문개요집
2016 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0