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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제2호
- 발행연도
- 2018.1
- 수록면
- 323 - 329 (7page)
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초록· 키워드
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In Escherichia coli, the transcription of genes related to metal homeostasis is activated by the presence of target metals. The promoter regions of those genes can be fused with reporter genes to generate whole-cell bioreporters (WCBs); these organisms sense the presence of target metals through reporter gene expression. However, the limited number of available promoters for sensing domains restricts the number of WCB targets. In this study, we have demonstrated an alternative method to generate novel WCBs, based on the notion that since the sensing mechanisms of WCBs are related to metal transportation systems, their properties can be modulated by disrupting metal homeostasis. Mutant E. coli strains were generated by deleting the znt-operon genes zntA, which encodes a zinc-export protein, and zntR, which encodes a znt-operon regulatory protein, to investigate the effects on the metal-sensing properties of WCBs. Deletion of zntA increased the sensitivity but abolished the selectivity of cadmiumsensing WCBs, whereas arsenic-sensing WCBs gained sensitivity toward cadmium. When zntR was deleted, cadmium-sensing WCBs lost the ability to detect cadmium, and this was recovered by introducing exogenous zntR. In addition, the metal-binding site of ZntR was genetically engineered to modulate metal selectivity. This study provides a valuable platform for the development of novel E. coli-based WCBs.
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참고문헌 (27)
참고문헌 신청
van de Mortel JE / 2006 / Large expression differences in genes for iron and zinc homeostasis, stress response, and lignin biosynthesis distinguish roots of Arabidopsis thaliana and the related metal hyperaccumulator Thlaspi caerulescens / Plant Physiol. 142:1127~1147
Clemens S / 2001 / Molecular mechanisms of plant metal tolerance and homeostasis / Planta 212:475~486
Canovas D / 2003 / Heavy metal tolerance and metal homeostasis in Pseudomonas putida as revealed by complete genome analysis / Environ. Microbiol. 5:1242~1256
Silver S / 1996 / Bacterial resistances to toxic metal ions - a review / Gene 179:9~19
Simpson ML / 1998 / Bioluminescent-bioreporter integrated circuits form novel whole-cell biosensors / Trends Biotechnol. 16:332~338
Clemens S / 2001 / Molecular mechanisms of plant metal tolerance and homeostasis / Planta 212:475~486
Canovas D / 2003 / Heavy metal tolerance and metal homeostasis in Pseudomonas putida as revealed by complete genome analysis / Environ. Microbiol. 5:1242~1256
Silver S / 1996 / Bacterial resistances to toxic metal ions - a review / Gene 179:9~19
Simpson ML / 1998 / Bioluminescent-bioreporter integrated circuits form novel whole-cell biosensors / Trends Biotechnol. 16:332~338
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