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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제2호
- 발행연도
- 2020.1
- 수록면
- 296 - 305 (10page)
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초록· 키워드
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Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.
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참고문헌 (38)
참고문헌 신청
ApSimon J / 2009 / The total synthesis of natural products / John Wiley & Sons, National Academies Press :317~326
Assaf S / 1997 / 1-Octen-3-ol and 13-hydroperoxylinoleate are products of distinct pathways in the oxidative breakdown of linoleic acid by Pleurotus pulmonarius / Enzyme Microb. Technol 21:484~490
Byers JA / 2004 / Avoidance of nonhost plants by a bark beetle, Pityogenes bidentatus, in a forest of odors / Naturwissenschaften 91:215~219
변세은 / 2009 / Functional Activation of Macrophages, Monocytes and Splenic Lymphocytes by Polysaccharide Fraction from Tricholoma matsutake / Archives of Pharmacal Research 32(11):1565~1572
Cronin DA / 1971 / The characterization of some mushroom volatiles / J. Sci. Food Agric 22:477~479
Assaf S / 1997 / 1-Octen-3-ol and 13-hydroperoxylinoleate are products of distinct pathways in the oxidative breakdown of linoleic acid by Pleurotus pulmonarius / Enzyme Microb. Technol 21:484~490
Byers JA / 2004 / Avoidance of nonhost plants by a bark beetle, Pityogenes bidentatus, in a forest of odors / Naturwissenschaften 91:215~219
변세은 / 2009 / Functional Activation of Macrophages, Monocytes and Splenic Lymphocytes by Polysaccharide Fraction from Tricholoma matsutake / Archives of Pharmacal Research 32(11):1565~1572
Cronin DA / 1971 / The characterization of some mushroom volatiles / J. Sci. Food Agric 22:477~479
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