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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 721 - 728 (8page)
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초록· 키워드
오류제보하기
Non-structural protein 1 (NS1) of influenza virus has been shown to inhibit the innate immune response by blocking the induction of interferon (IFN). In this study, we isolated two single-stranded RNA aptamers specific to NS1 with Kd values of 1.62 ± 0.30 nM and 1.97 ± 0.27 nM, respectively, using a systematic evolution of ligand by exponential enrichment (SELEX) procedure. The selected aptamers were able to inhibit the interaction of NS1 with tripartite motif-containing protein 25 (TRIM25), and suppression of NS1 enabled retinoic acid inducible gene I (RIG-I) to be ubiquitinated regularly by TRIM25. Additional luciferase reporter assay and quantitative real-time PCR (RT-PCR) experiments demonstrated that suppression of NS1 by the selected aptamers induced IFN production. It is noted that viral replication was also inhibited through IFN induction in the presence of the selected aptamers. These results suggest that the isolated aptamers are strongly expected to be new therapeutic agents against influenza infection.
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참고문헌 (27)
참고문헌 신청
Aramini, J. M. / 2011 / Dimer interface of the effector domain of non-structural protein 1 from influenza A virus : an interface with multiple functions / J. Biol. Chem 286:26050~26060
Basu, D. / 2009 / Novel influenza virus NS1 antagonists block replication and restore innate immune function / J. Virol 83:1881~1891
Bornholdt, Z. A. / 2006 / X-ray structure of influenza virus NS1 effector domain / Nat. Struct. Mol. Biol 13:559~560
Brody, E. N. / 2000 / Aptamers as therapeutic and diagnostic agents / J. Biotechnol 74:5~13
Cheng, C. / 2008 / Potent inhibition of human influenza H5N1 virus by oligonucleotides derived by SELEX / Biochem. Biophys. Res. Commun 366:670~674
Basu, D. / 2009 / Novel influenza virus NS1 antagonists block replication and restore innate immune function / J. Virol 83:1881~1891
Bornholdt, Z. A. / 2006 / X-ray structure of influenza virus NS1 effector domain / Nat. Struct. Mol. Biol 13:559~560
Brody, E. N. / 2000 / Aptamers as therapeutic and diagnostic agents / J. Biotechnol 74:5~13
Cheng, C. / 2008 / Potent inhibition of human influenza H5N1 virus by oligonucleotides derived by SELEX / Biochem. Biophys. Res. Commun 366:670~674
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