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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제11호
- 발행연도
- 2017.1
- 수록면
- 2,037 - 2,043 (7page)
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초록· 키워드
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The surface protein hemagglutinin (HA) mediates the attachment of influenza virus to host cells containing sialic acid and thus facilitates viral infection. Therefore, HA is considered as a good target for the development of diagnostic tools for influenza virus. Previously, we reported the isolation of single-stranded aptamers that can distinguish influenza subtype H1 from H5. In this study, we describe a method for the selective electrical detection of H1 using the isolated aptamer as a molecular probe. After immobilization of the aptamer on Si wafer, enzyme-linked immunosorbent assay (ELISA) and field emission scanning electron microscopy (FE-SEM) showed that the immobilized aptamer bound specifically to the H1 subtype but not to the H5 subtype. Assessment by cyclic voltammetry (CV) also demonstrated that the immobilized aptamer on the indium thin oxide-coated surface was specifically bound to the H1 subtype only, which was consistent with the ELISA and FE-SEM results. Further measurement of CV using various amounts of H1 subtype provided the detection limit of the immobilized aptamer, which showed that a nanomolar scale of target protein was sufficient to produce the signal. These results indicated that the selected aptamer can be an effective probe for distinguishing the subtypes of influenza viruses by monitoring current changes.
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참고문헌 (24)
참고문헌 신청
Arya SK / 2007 / Biosensor for total cholesterol estimation using N-(2-aminoethyl)-3-aminopropyltrimethoxysilane self-assembled monolayer / Anal. Bioanal. Chem 389 : 2235 ~
Balamurugan S / 2008 / Surface immobilization methods for aptamer diagnostic applications / Anal. Bioanal. Chem 390 : 1009 ~ 1021
Cherian T / 1994 / Use of PCR-enzyme immunoassay for identification of influenza A virus matrix RNA in clinical samples negative for cultivable virus / J. Clin. Microbiol 32
Deng YM / 2011 / Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing / PLoS One 6 : e23400 ~
Gamblin SJ / 2010 / Influenza hemagglutinin and neuraminidase membrane glycoproteins / J. Biol. Chem 285 : 28403 ~ 28409
Balamurugan S / 2008 / Surface immobilization methods for aptamer diagnostic applications / Anal. Bioanal. Chem 390 : 1009 ~ 1021
Cherian T / 1994 / Use of PCR-enzyme immunoassay for identification of influenza A virus matrix RNA in clinical samples negative for cultivable virus / J. Clin. Microbiol 32
Deng YM / 2011 / Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing / PLoS One 6 : e23400 ~
Gamblin SJ / 2010 / Influenza hemagglutinin and neuraminidase membrane glycoproteins / J. Biol. Chem 285 : 28403 ~ 28409
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