지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 1 - 22 (22page)
이용수
초록· 키워드
오류제보하기
To study whether TGF-β1/IL-11/MEK/ERK (TIME) signaling mediates senescence-associated pulmonary fibrosis (SAPF) in Bmi-1-deficient (Bmi-1−/−) mice and determines the major downstream mediator of Bmi-1 and crosstalk between p16INK4a and reactive oxygen species that regulates SAPF, phenotypes were compared among 7-week-old p16INK4a and Bmi-1 double-knockout, N-acetylcysteine (NAC)-treated Bmi-1−/−, Bmi-1−/−, and wild-type mice. Pulmonary fibroblasts and alveolar type II epithelial (AT2) cells were used for experiments. Human pulmonary tissues were tested for type Ι collagen, α-smooth muscle actin (α-SMA), p16INK4a, p53, p21, and TIME signaling by using enzyme-linked immunosorbent assay (ELISA). Our results demonstrated that Bmi-1 deficiency resulted in a shortened lifespan, ventilatory resistance, poor ventilatory compliance, and SAPF, including cell senescence, DNA damage, a senescence-associated secretory phenotype and collagen overdeposition that was mediated by the upregulation of TIME signaling. The signaling stimulated cell senescence, senescence-related secretion of TGF-β1 and IL-11 and production of collagen 1 by pulmonary fibroblasts and the epithelial-to-mesenchymal transition of AT2 cells. These processes were inhibited by anti-IL-11 or the MEK inhibitor PD98059. NAC treatment prolonged the lifespan and ameliorated pulmonary dysfunction and SAPF by downregulating TIME signaling more than p16INK4a deletion by inhibiting oxidative stress and DNA damage and promoting ubiquitinproteasome degradation of p16INK4a and p53. Cytoplasmic p16INK4a accumulation upregulated MEK/ERK signaling by inhibiting the translocation of pERK1/2 (Thr202/Tyr204) from the cytoplasm to the nucleus in senescent fibroblasts. The accumulation of collagen 1 and α-SMA in human lungs accompanied by cell senescence may be mediated by TIME signaling. Thus, this signaling in aging fibroblasts or AT2 cells could be a therapeutic target for preventing SAPF.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Cellular senescence, aging, and age-related disease: Special issue of BMB Reports in 2019
BMB Reports
2019 .01
-
Cellular senescence: a promising strategy for cancer therapy
BMB Reports
2019 .01
-
Juxtacrine regulation of cellular senescence
BMB Reports
2019 .01
-
MicroRNA controls of cellular senescence
BMB Reports
2018 .01
-
From cell senescence to age-related diseases: differential mechanisms of action of senescence-associated secretory phenotypes
BMB Reports
2015 .01
-
Particulate matter-induced senescence of skin keratinocytes involves oxidative stress-dependent epigenetic modifications
Experimental and Molecular Medicine
2019 .01
-
Annexin A2 as a regulator of the cellular senescence and apoptosis through p21
한국생물공학회 학술대회
2015 .04
-
Disruption of nucleocytoplasmic trafficking as a cellular senescence driver
Experimental and Molecular Medicine
2021 .06
-
Peroxiredoxin I participates in the protection of reactive oxygen species-mediated cellular senescence
BMB Reports
2017 .01
-
Metabolic features and regulation in cell senescence
BMB Reports
2019 .01
-
Defining regorafenib as a senomorphic drug: therapeutic potential in the age-related lung disease emphysema
Experimental and Molecular Medicine
2023 .04
-
Endothelial senescence in vascular diseases: current understanding and future opportunities in senotherapeutics
Experimental and Molecular Medicine
2023 .01
-
잉크젯 잉크의 수명예측을 위한 광열화 가속시험법에 관한 연구
신뢰성응용연구
2015 .09
-
Interleukin-11 signaling underlies fibrosis, parenchymal dysfunction, and chronic inflammation of the airway
Experimental and Molecular Medicine
2020 .12
-
Alternative Polyadenylation(APA)-mediated 3’UTR Lengthening Contributes to Cellular Senescence
한국생물공학회 학술대회
2023 .04
-
Global transcriptional downregulation of TREX and nuclear trafficking machinery as pan-senescence phenomena: evidence from human cells and tissues
Experimental and Molecular Medicine
2020 .08
-
Cellular senescence in cancer
BMB Reports
2019 .01
-
Eupatilin Inhibits Hepatic Fibrosis by Blocking TGF-β1 /non-Smad Signaling
한국생물공학회 학술대회
2018 .04
-
YAP1 inhibits the senescence of alveolar epithelial cells by targeting Prdx3 to alleviate pulmonary fibrosis
Experimental and Molecular Medicine
2024 .07
이 논문의 저자 정보
최근 본 자료
댓글(0)
0