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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.10
- 수록면
- 600 - 605 (6page)
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초록· 키워드
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Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositions of the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years, the aerial parts of members of the Panax genus have received great attention from natural product chemists as producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation of novel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro.
Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells.
Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside LS₁ (1) and 5,6-didehydroginsenoside Rg₃ (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with IC<SUB>50</SUB> of 37.38 µM compared with that of NG-monomethyl-L-arginine (IC<SUB>50</SUB> = 90.76 µM). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin E₂ and tumor necrosis factor-a. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.
Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells.
Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside LS₁ (1) and 5,6-didehydroginsenoside Rg₃ (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with IC<SUB>50</SUB> of 37.38 µM compared with that of NG-monomethyl-L-arginine (IC<SUB>50</SUB> = 90.76 µM). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin E₂ and tumor necrosis factor-a. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (38)
참고문헌 신청
오지연 / 2014 / Investigation of ginsenosides in different tissues after elicitor treatment in Panax ginseng / Journal of Ginseng Research 38(4):270~277
Cheng YJ / 2011 / Two-step preparation of ginsenoside Re, Rb1, Rc and Rb2 from the root of Panax ginseng by high-performance counter-current chromatography / Sep Purif Technol 77:347~354
Xie JT / 2004 / American ginseng leaf : ginsenoside analysis and hypoglycemic activity / Pharmacol Res 49:113~117
Li TSC / 1996 / Ginsenosides in roots and leaves of American ginseng / J Agric Food Chem 44:717~720
Leung KW / 2006 / Ginsenoside-Rg1 induces vascular endothelial growth factor expression through the glucocorticoid receptorrelated phosphatidylinositol 3-kinase/akt and b-catenin/T-cell factordependent pathway in human endothelial cells / J Biol Chem 281:36280~36288
Cheng YJ / 2011 / Two-step preparation of ginsenoside Re, Rb1, Rc and Rb2 from the root of Panax ginseng by high-performance counter-current chromatography / Sep Purif Technol 77:347~354
Xie JT / 2004 / American ginseng leaf : ginsenoside analysis and hypoglycemic activity / Pharmacol Res 49:113~117
Li TSC / 1996 / Ginsenosides in roots and leaves of American ginseng / J Agric Food Chem 44:717~720
Leung KW / 2006 / Ginsenoside-Rg1 induces vascular endothelial growth factor expression through the glucocorticoid receptorrelated phosphatidylinositol 3-kinase/akt and b-catenin/T-cell factordependent pathway in human endothelial cells / J Biol Chem 281:36280~36288
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UCI(KEPA) : I410-ECN-0101-2020-524-000504839