지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.12
- 수록면
- 398 - 406 (9page)
이용수
초록· 키워드
오류제보하기
Cancer immunotherapy using gene-modified tumor cells is safe and customized cancer treatment method. In this study, we made gene-modified tumor cells by transferring costimulatory molecules, 4-1BBL and OX40L, into tumor cells using lentivirus vector, and identified anti-cancer effect of gene-modified tumor cells in CT26 mouse colorectal tumor model. We construct pLVX-puro-4-1BBL, -OX40L vector for lentivirus production and optimized the transfection efficiency and transduction efficiency. The transfection efficiency is maximal at DNA:cationic polymer = 1:0.5 and DNA 2 μg for lentivirus production. Then, the lentiviral including 4-1BBL and OX40L was used to deliver CT26 mouse tumor cells to establish optimal delivery conditions according to the amount of virus. The transduction efficiency is maximal at 500 μL volume of lentiviral stock without change in cell shape or growth rate. CT26-4-1BBL, CT26-OX40L significantly inhibited the tumor growth compare with CT26-WT or CT26-β-gal cell line. These data showed the possibility the use of genetically modified tumor cells with costimulatory molecule as cancer immunotherapy agent.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (13)
참고문헌 신청
Eager R / 2005 / Gm-csf gene-transduced tumor vaccines / Mol Ther 12:18~27
Perica K / 2015 / Adoptive t cell immunotherapy for cancer / Rambam Maimonides Med J 6:e0004
Si C / 2017 / In vivo antitumor activity evaluation of cancer vaccines prepared by various antigen forms in a murine hepatocellular carcinoma model / Oncol Lett 14:7391~7397
Srivatsan S / 2014 / Allogeneic tumor cell vaccines: The promise and limitations in clinical trials / Hum Vaccin Immunother 10:52~63
Stephan MT / 2007 / T cell-encoded cd80 and 4-1bbl induce auto- and transcostimulation, resulting in potent tumor rejection / Nat Med 13:1440~1449
Perica K / 2015 / Adoptive t cell immunotherapy for cancer / Rambam Maimonides Med J 6:e0004
Si C / 2017 / In vivo antitumor activity evaluation of cancer vaccines prepared by various antigen forms in a murine hepatocellular carcinoma model / Oncol Lett 14:7391~7397
Srivatsan S / 2014 / Allogeneic tumor cell vaccines: The promise and limitations in clinical trials / Hum Vaccin Immunother 10:52~63
Stephan MT / 2007 / T cell-encoded cd80 and 4-1bbl induce auto- and transcostimulation, resulting in potent tumor rejection / Nat Med 13:1440~1449
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Anti-tumor Effect of 4-1BBL Modified Tumor Cells as Preventive and Therapeutic Vaccine
대한의생명과학회지
2022 .12
-
CD8-dependent Tumor Growth Inhibition by Tumor Cells Genetically Modified with 4-1BBL
대한의생명과학회지
2021 .12
-
Mouse tumor model and overview of surgical tumor model
한국실험동물학회 학술발표대회 논문집
2020 .02
-
Solid Tumors in Children and Adolescents
Journal of Korean Medical Science
2018 .01
-
Established tumor model in nude mice using canine tumor cells, ChMP
한국실험동물학회 학술발표대회 논문집
2021 .07
-
Optomizing Transfection Efficiency of Cervical Cancer Cells Transfected by Cationic Liposomes Lipofectamine<sup>TM</sup>2000
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Multifocality and Bilaterality of Papillary Thyroid Microcarcinoma
Clinical and Experimental Otorhinolaryngology
2015 .01
-
Anti-tumor effects of yeast extract on colorectal cancer cells
대한체질인류학회 학술대회 연제 초록
2019 .05
-
Reprogramming the tumor microenvironment with biotechnology
생체재료학회지
2023 .03
-
Tumor Cells and Cancer-Associated Fibroblasts: A Synergistic Crosstalk to Promote Thyroid Cancer
Endocrinology and Metabolism
2020 .01
-
Murine Mammary Carcinoma Induces Chronic Systemic Inflammation and Immunosuppression in BALB/c Mice
Journal of Breast Cancer
2022 .06
-
Radiation-induced immune responses: mechanisms and therapeutic perspectives
Blood Research
2016 .01
-
Genetically modified mouse models in cancer research
한국실험동물학회 학술발표대회 논문집
2017 .02
-
DLK1 suppresses melanoma growth by shaping tumor immune microenvironment
한국실험동물학회 학술발표대회 논문집
2021 .07
-
Anti-tumor effects and mechanism of tannin-enriched extract of Ecklonia cava in syngeneic model using CT26 (colon carcinoma) cells
한국실험동물학회 학술발표대회 논문집
2021 .01
-
Insights into granulosa cell tumors using spontaneous or genetically engineered mouse models
Clinical and experimental reproductive medicine : CERM
2016 .01
-
전산화단층촬영에서 안면신경 해부학적 지표와 교차하는 이하선 종양의 위치예측
대한이비인후-두경부외과학회지
2019 .01
-
Role of notch signaling in tumor associated macrophages
한국실험동물학회 학술발표대회 논문집
2021 .07
-
Circulating Tumor Cell and Cell-free Circulating Tumor DNA in Lung Cancer
전남의대학술지
2016 .01
-
Inducible Mouse Models for Cancer Drug Target Validation
대한암예방학회지
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2020-510-000240514