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The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro MTA® (RM), EZ-SealTM (EZ) and ENDOCEM Zr® (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation: RM > EN > EZ p( = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.
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참고문헌 (28)
참고문헌 신청
Fuks AB / 2008 / Vital pulp therapy with new materials for primary teeth : new directions and treatment perspectives / J Endod 34:S18~S24
Rodd HD / 2006 / Pulp therapy for primary molars / Int J Paediatr Dent 16(Suppl 1):15~23
Gandolfi MG / 2015 / Calcium silicate/calcium phosphate biphasic cements for vital pulp therapy : chemical-physical properties and human pulp cells response / Clin Oral Investig 19:2075~2089
Prati C / 2015 / Calcium silicate bioactive cements : biological perspectives and clinical applications / Dent Mater 31:351~370
Han L / 2015 / Evaluation of calcium-releasing and apatite-forming abilities of fast-setting calcium silicatebased endodontic materials / Int Endod J 48:124~130
Rodd HD / 2006 / Pulp therapy for primary molars / Int J Paediatr Dent 16(Suppl 1):15~23
Gandolfi MG / 2015 / Calcium silicate/calcium phosphate biphasic cements for vital pulp therapy : chemical-physical properties and human pulp cells response / Clin Oral Investig 19:2075~2089
Prati C / 2015 / Calcium silicate bioactive cements : biological perspectives and clinical applications / Dent Mater 31:351~370
Han L / 2015 / Evaluation of calcium-releasing and apatite-forming abilities of fast-setting calcium silicatebased endodontic materials / Int Endod J 48:124~130
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