지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한병리학회 Journal of Pathology and Translational Medicine Journal of Pathology and Translational Medicine 제53권 제5호
- 발행연도
- 2019.1
- 수록면
- 317 - 326 (10page)
이용수
초록· 키워드
오류제보하기
Background: Single staining is commonly performed for practical pathologic diagnoses. However, this method is limited in its ability to specify cellular morphology and immunophenotype and often requires consumption of limited tissue. This study aimed to describe an optimized protocol for multiple in situ hybridization (ISH) and immunohistochemistry (IHC). Methods: The quality of multistaining was evaluated by carefully changing each step of ISH and IHC in an angioimmunoblastic T-cell lymphoma (AITL) case on a Ventana Bench-Mark XT automated immunostainer. The optimized protocols were also performed using another immunostainer and in 15 cases of five Epstein-Barr virus (EBV)–associated malignancies using formalin-fixed paraffin-embedded tissue. Results: The quality of various ISH-IHC staining protocols was semi-quantitatively evaluated. The best EBV-encoded RNA (EBER)-ISH/double IHC staining quality, equivalent to single staining, was obtained using the following considerations: initial EBER-ISH application, use of protease and antigen retrieval reagent (cell conditioning 1 [CC1] treatment time was minimized due to impact on tissue quality), additional baking/deparaffinization not needed, and reduced dilution ratio and increased reaction time for primary antibody compared with single immunostaining. Furthermore, shorter second CC1 treatment time yielded better results. Multiple staining was the best quality in another immunostainer and for different types of EBV-associated malignancies when it was performed in the same manner as for the Ventana BenchMark XT as determined for AITL. Conclusions: EBER-ISH and double IHC could be easily used in clinical practice with currently available automated immunostainers and adjustment of reagent treatment time, dilution ratio, and antibody reaction time.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Detection of EBV-encoded Small RNA from Diffuse Large B-cell Lymphoma Patients by RT-PCR Method
대한의생명과학회지
2015 .12
-
Epstein-Barr Virus-Positive Diffuse Large B-Cell Lymphoma: is it different between Over and Under 50 Years of Age?
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Overexpression of HER2/HER3 and clinical feature of ovarian cancer
Journal of Gynecologic Oncology
2019 .01
-
Metachronous extranodal natural killer/T-cell lymphoma of nasal type and primary testicular lymphoma
Journal of Yeungnam Medical Science
2021 .01
-
Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips
Journal of Pathology and Translational Medicine
2016 .01
-
Chromogenic In Situ Hybridisation Test for Breast Cancer Patients with Equivocal IHC Results - a Study from Iran
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Bone Marrow Involvement of Epstein-Barr Virus-Positive Large B-Cell Lymphoma in a Patient with Angioimmunoblastic T-Cell Lymphoma
Annals of Laboratory Medicine
2018 .01
-
Detection of Gene Amplification by Multiplex Ligation-Dependent Probe Amplification in Comparison with In Situ Hybridization and Immunohistochemistry
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
The Prognostic Role of Circulating Epstein-Barr Virus DNA Copy Number in Angioimmunoblastic T-Cell Lymphoma Treated with Dose-Adjusted EPOCH
Cancer Research and Treatment
2019 .01
-
Diagnostic Performance and Comparative Evaluation of the Architect, Liaison, and Platelia Epstein-Barr Virus Antibody Assays
Annals of Laboratory Medicine
2018 .01
-
Evaluating the Efficacy of a Formalin Alternative in Fixing Pathological Tissues for Histological and Molecular Diagnoses
대한의생명과학회지
2023 .03
-
Epstein-Barr Virus-Associated Vesiculopapular Eruption on the Face of a Patient with Natural Killer T Cell Lymphoma
Annals of Dermatology
2017 .01
-
A machine-learning expert-supporting system for diagnosis prediction of lymphoid neoplasms using a probabilistic decision-tree algorithm and immunohistochemistry profile database
Journal of Pathology and Translational Medicine
2020 .01
-
Usefulness of BRAF VE1 immunohistochemistry in non–small cell lung cancers: a multi-institutional study by 15 pathologists in Korea
Journal of Pathology and Translational Medicine
2022 .11
-
Unsuspected systemic Epstein-Barr virus–positive T-cell lymphoma of childhood diagnosed at autopsy in a potential homicide case
Journal of Pathology and Translational Medicine
2023 .03
-
Predictive Value of IHC4 Score for Pathological Response to Neoadjuvant Chemotherapy in Hormone Receptor-Positive Breast Cancer
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Role of Helicobacter pylori Immunohistochemistry in the Histopathological Assessment of Inflamed Endoscopic Gastric Biopsies
Korean Journal of Helicobacter Upper Gastrointestinal Research
2024 .03
-
한국인의 Epstein-Barr Virus 항체 양성률 변화양상에 대한 분석: 단일기관 연구
Pediatric Infection and Vaccine
2020 .01
-
병리 진단 과정 및 결과 해석 - 면역조직화학염색을 중심으로
대한내과학회지
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0