지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한정신약물학회 Clinical Psychopharmacology and Neuroscience Clinical Psychopharmacology and Neuroscience 제17권 제3호
- 발행연도
- 2019.1
- 수록면
- 438 - 442 (5page)
이용수
초록· 키워드
오류제보하기
Objective: Until recently, riluzole was the only drug licensed for amyotrophic lateral sclerosis (ALS). In spite of its efficacy, the mechanism of action remains elusive, and both blocking of glutamate release and antioxidant properties have been postulated. Here we characterized human SH-SY5Y neuroblastoma cell lines, taking advantage of their insensitivity to excitotoxic insults, in order to selectively assess the presence of a direct antioxidant effect of riluzole. Methods: SH-SY5Y cells, either parental or overexpressing the G93A SOD1 mutation, were exposed for 24 hours to the selected stimuli. Results: Riluzole (1‒10 M) was able to counteract the effects of H2O2 exposure (200 M/24 hr), limiting both cell death and whole-cell reactive oxygen species (ROS) increase. The same experiments were repeated using SH-SY5Y cells carrying the familial ALS-related G93A-SOD1 mutation and constitutively expressing two-fold increased whole-cell ROS levels with respect to wild-type cells: riluzole was ineffective in this paradigm. Analogously, riluzole was ineffective in preventing cell death induced by exposing SH-SY5Y cells to 3-morpholino-sydnonimine (SIN-1, 1.5 mM/24 hr), a reactive nitrogen species (RNS) donor. Conclusion: Our data support a direct antioxidant action of riluzole. Furthermore, the lack of efficacy of riluzole observed in the SOD1 cell model mirrors the lack of efficacy already demonstrated in cognate mouse models of ALS, plausibly reflecting differences in the underlying pathogenic mechanisms. Finally, riluzole inefficacy against nitrosative stress might support the idea that a combined therapeutic intervention may result more effective in ALS patients, as in the case of co-administration of edaravone, a drug known to reduce RNS.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Miller RG / 2012 / Riluzole for amyotrophic lateral sclerosis (ALS)/motor neuron disease (MND) / Cochrane Database Syst Rev (3):CD001447
Wokke J / 1996 / Riluzole / Lancet 348:795~799
Azbill RD / 2000 / Riluzole increases high-affinity glutamate uptake in rat spinal cord synaptosomes / Brain Res 871:175~180
Koh JY / 1999 / Antioxidative and proapoptotic effects of riluzole on cultured cortical neurons / J Neurochem 72:716~723
Storch A / 2000 / Protective effects of riluzole on dopamine neurons : involvement of oxidative stress and cellular energy metabolism / J Neurochem 75:2259~2269
Wokke J / 1996 / Riluzole / Lancet 348:795~799
Azbill RD / 2000 / Riluzole increases high-affinity glutamate uptake in rat spinal cord synaptosomes / Brain Res 871:175~180
Koh JY / 1999 / Antioxidative and proapoptotic effects of riluzole on cultured cortical neurons / J Neurochem 72:716~723
Storch A / 2000 / Protective effects of riluzole on dopamine neurons : involvement of oxidative stress and cellular energy metabolism / J Neurochem 75:2259~2269
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
근위축측삭경화증에 대한 치료약물 임상시험 현황
Annals of Clinical Neurophysiology
2015 .01
-
Propolis Inhibits Neurite Outgrowth in Differentiating SH-SY5Y Human Neuroblastoma Cells
Toxicological Research
2016 .07
-
A Novel F45S SOD1 Mutation in Amyotrophic Lateral Sclerosis Coexisting with Bullous Pemphigoid
Journal of Clinical Neurology
2015 .01
-
Likely pathogenic FIG4 related amyotrophic lateral sclerosis patient who correlated with clinical, imaging and neuropsychological studies
Annals of Clinical Neurophysiology
2020 .01
-
Neuroprotective effects of mulberry fruit on H2O2-induced oxidative neuronal damage via MAPK/NF-κB signaling pathway in SH-SY5Y neuroblastoma cells
한국실험동물학회 학술발표대회 논문집
2020 .07
-
근위축성 측삭경화증의 한방치료에 관한 문헌적 고찰연구
대한침구의학회지
2015 .01
-
Immune Correlates of Resistance to Trichinella spiralis Reinfection in Mice
Parasites, Hosts and Diseases
2016 .10
-
Clonal Expansion of Allergen-specific CD4+ T Cell in the Lung in the Absence of Lymph Nodes
Immune Network
2017 .06
-
MiR-212 Attenuates MPP+-Induced Neuronal Damage by Targeting KLF4 in SH-SY5Y Cells
Yonsei Medical Journal
2018 .01
-
Comparison of the Effects of 13-cis Retinoic Acid and Melatonin on the Viabilities of SH-SY5Y Neuroblastoma Cell Line
대한신경외과학회지
2015 .01
-
7,8,4'-Trihydroxyisoflavone, a Metabolized Product of Daidzein, Attenuates 6-Hydroxydopamine-Induced Neurotoxicity in SH-SY5Y Cells
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Neuroprotective Effect of Root Extracts of Berberis Vulgaris (Barberry) on Oxidative Stress on SH-SY5Y Cells
Journal of Pharmacopuncture
2022 .09
-
운동치료가 근위축성측삭경화증 환자 삶의 질에 미치는 영향: 예비연구
대한근전도 전기진단의학회지
2018 .01
-
Regulation of Th2 Cell Immunity by Dendritic Cells
Immune Network
2016 .02
-
Uncommon coexistence of myasthenia gravis and amyotrophic lateral sclerosis
Annals of Clinical Neurophysiology
2019 .01
-
Neuregulin-1 Protects Neuronal Cells Against Damage due to CoCl2-Induced Hypoxia by Suppressing Hypoxia-Inducible Factor-1α and P53 in SH-SY5Y Cells
International Neurourology Journal
2019 .01
-
Prognostic Role of Serum Levels of Uric Acid in Amyotrophic Lateral Sclerosis
Journal of Clinical Neurology
2015 .01
-
Heterogeneity of Islet Cells during Embryogenesis and Differentiation
Diabetes and Metabolism Journal
2023 .03
-
Calcium Signaling of Lysophosphatidylethanolamine through LPA<sub>1</sub> in Human SH-SY5Y Neuroblastoma Cells
Biomolecules & Therapeutics(구 응용약물학회지)
2017 .01
-
A case report on the use of processed Glycyrrhiza uralensis extract and Korean medical treatment for a patient with Amyotrophic Lateral Sclerosis(ALS)
대한침구의학회지
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0